| 黄芪多糖对人乳腺癌MDA-MB-231裸鼠移植瘤生长及肿瘤相关凋亡蛋白的影响 |
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| 引用本文： | 谢荣丹,孙少伯,何建新,董静凝,龙凤.黄芪多糖对人乳腺癌MDA-MB-231裸鼠移植瘤生长及肿瘤相关凋亡蛋白的影响[J].中国实验方剂学杂志,2019,25(16):37-43. |
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| 作者姓名： | 谢荣丹 孙少伯 何建新 董静凝 龙凤 |
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| 作者单位： | 甘肃中医药大学 中西医结合研究所, 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室, 兰州 730000,甘肃中医药大学 中西医结合研究所, 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室, 兰州 730000,甘肃中医药大学 中西医结合研究所, 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室, 兰州 730000,甘肃中医药大学 中西医结合研究所, 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室, 兰州 730000,甘肃中医药大学 中西医结合研究所, 甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室, 兰州 730000 |
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| 基金项目： | 国家自然科学基金项目（81503377）；甘肃省高等学校基本科研业务费专项（998602140604） |
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| 摘 要： | 目的:观察黄芪多糖(Astragalus polysaccharides,APS)对人乳腺癌MDA-MB-231裸鼠移植瘤的生长抑制作用及其对肿瘤细胞凋亡的影响,研究APS抑制三阴性乳腺癌MDA-MB-231生长、诱导其凋亡的作用及其分子机制。方法:通过将人乳腺癌细胞MDA-MB-231接种于BALB/c-nu雌性裸鼠右侧腋窝皮下建立人乳腺癌裸鼠移植瘤模型; 18只乳腺癌荷瘤裸鼠随机分为3组:模型组(每天予生理盐水),APS低、高剂量组(200,400 mg·kg~(-1)),每组6只;每天灌胃给药200μL,连续21 d。实验结束,观察比较APS低、高剂量组与模型组荷瘤鼠肿瘤质量、瘤体体积的变化,计算肿瘤抑制率;苏木精-伊红(HE)染色观察瘤体组织细胞形态学变化;原位末端标记法(TUNEL)检测乳腺癌肿瘤组织的凋亡;蛋白免疫印迹法检测肿瘤组织中B细胞淋巴瘤/白血病-2蛋白(Bcl-2),Bcl-2相关X蛋白(Bax)、半胱天冬氨酸蛋白酶(Caspase)等凋亡相关蛋白表达。结果:与模型组比较,APS低、高剂量组中的乳腺癌肿瘤体积、瘤重减小(P 0. 05,P 0. 01),其肿瘤抑制率分别为37. 9%,57. 57%; HE染色观察APS干预组肿瘤组织均呈现明显形态学改变,组织细胞出现凋亡状态; TUNEL染色显示APS组肿瘤组织细胞凋亡率增加;蛋白免疫印迹法发现,与模型组比较,APS低、高剂量组肿瘤组织中Bcl-2蛋白水平下降(P 0. 05,P 0. 01),Bax,Caspase-9,Caspase-7蛋白表达水平上调(P 0. 05,P 0. 01)。结论:APS能有效抑制MDA-MB-231乳腺癌裸鼠移植瘤生长,诱导人乳腺癌MDA-MB-231细胞发生凋亡,其作用机制与APS影响乳腺癌细胞中凋亡相关蛋白Bcl-2,Bax,Caspase-9,Caspase-7蛋白表达水平有关。
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| 关 键 词： | 黄芪多糖 乳腺癌 MDA-MB-231裸鼠移植瘤 肿瘤抑制 凋亡 |
| 收稿时间： | 2019/1/25 0:00:00 |
Effect of Astragalus Polysaccharide on Growth and Tumor-related Apoptosis Protein of Human Breast Cancer MDA-MB-231 Transplanted Tumor in Nude Mice |
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| XIE Rong-dan,SUN Shao-bo,HE Jian-xin,DONG Jing-ning and LONG Feng.Effect of Astragalus Polysaccharide on Growth and Tumor-related Apoptosis Protein of Human Breast Cancer MDA-MB-231 Transplanted Tumor in Nude Mice[J].China Journal of Experimental Traditional Medical Formulae,2019,25(16):37-43. |
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| Authors: | XIE Rong-dan SUN Shao-bo HE Jian-xin DONG Jing-ning and LONG Feng |
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| Institution: | Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Institute of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China,Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Institute of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China,Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Institute of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China,Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Institute of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China and Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Institute of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China |
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| Abstract: | |
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| Keywords: | astragalus polysaccharide breast cancer MDA-MB-231 transplanted tumor in nude mice tumor inhibition apoptosis |
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