海南地区类鼻疽伯克霍尔德菌的多位点序列分型

目的 研究海南省类鼻疽伯克霍尔德菌多位点序列类型(STs)多态性,了解该省与其他类鼻疽流行地区菌株间的遗传背景差异方法 选取7个管家基因位点ace、glt B、gmh D、lep A、lip A、nar K、ndh进行PCR扩增、测序,与MLST数据库中的等位基因序列进行比对,确定菌株的序列型结果 18株类鼻疽伯克霍尔德菌属于12个不同的STs,其中ST50有4株,检出率占22.2%,ST58、ST232、ST1094各检出2株,发现一株新STs命名为ST1676结论 海南省类鼻疽伯克霍尔德菌具有高度遗传多样性,gmhD是7个管家基因中变异最大的等位基因,表明gmhD突变可能是该地区菌株STs多样性的原因。

Multilocus sequence typing of Burkholderia pseudomallei collected in Hainan,China

This work aims to study the polymorphism of the multilocus sequence typing of 18 Burkholderia pseudomallei in Hainan province and to understand the difference in genetic background of the isolates in other endemic areas. Fragments of seven housekeeping genes including ace,glt B,gmh D,lep A,lip A,nar K and ndh were amplified and the obtained sequences were compared using MLST website. 18 isolates of B.pseudomallei were used and it was found 12 STs were identified, among which a new ST1676 was identified in this study. Among these STs, ST50 recovered 22.2% (4 cases ), ST58 (2), ST232 (2), ST1094 (2), and one case of each remaining STs. Overall these result, we concluded Hainan province B.pseudomallei is highly diverse. GmhD is the most variable allele of the seven housekeeping genes, suggested that variation in this allele may be responsible for the high genetic diversity of STs observed in this study.