高山红景天细胞悬浮培养生长和产物积累动力学

目的研究高山红景天悬浮细胞生长、红景天多糖和红景天苷合成动力学特征,为培养过程的优化控制提供理论依据。方法考察1个培养周期内高山红景天细胞的鲜重、干重和红景天苷、红景天多糖的含量,绘制生长曲线、生长速率曲线和比生长速率曲线。结果高山红景天细胞悬浮培养的生长周期约为16 d,0~4 d为细胞的延滞期,4~12 d为对数期,13~15 d为稳定期,在第14天时细胞鲜、干质量达到最大,分别为266.13 g.L-1和13.41 g.L-1;红景天苷在培养的第12天达到最大,其质量分数为0.585%;第10天时红景天多糖的含量达到最大,其(质量分数为13.824%)。结论确定细胞生长和产物积累的变化规律,可以提高生产效率,为培养过程的优化控制提供理论依据。

Growth of cell suspension cuture and kinetics of product accumulation in Rhodiola sachalinensis A.Bor.

Objective To investigate the kinetics of cell growth, Rhodiola sachalinensis polysaccharide and salidroside in Rhodiola sachalinensis A.Bor. Method The fresh weight, dry weight, the content of salidroside and RSP were investigated in a cell cycle, and the growth curve, the growth rate curve and the specific growth rate curve were drew. Result The growth cycle of R.sachalinesis cell suspension culture was 16 days. The lag phase was 0-4 d, the log phase was 4-12 d, the stable phase was 13-15 d, the decline phase was 16d. The highest fresh weight and dry weight were achieved on the 14th day, and reached 266.13 g·L-1and 13.41 g·L-1 respectively. Salidroside content reached the maximum 0.585%(w) on the 12th day. RSP content achieved the highest value 13.824%(w) on the 10th day. Conclusion Determine the variation law of cell growth and product accumulation, in order to raise the productive efficacy and to provide the theory evidence for optimizing controls of culture process.