| 甘露醇对骨髓间充质干细胞治疗血管性痴呆大鼠行为学及海马胆碱能系统的影响 |
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| 引用本文： | 张泰鹏,莫雪安,刘金萍,杨龙秀,陈志.甘露醇对骨髓间充质干细胞治疗血管性痴呆大鼠行为学及海马胆碱能系统的影响[J].中风与神经疾病杂志,2011,28(4). |
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| 作者姓名： | 张泰鹏 莫雪安 刘金萍 杨龙秀 陈志 |
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| 作者单位： | 广西医科大学第一附属医院神经内科,广西,南宁,530021 |
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| 基金项目： | 广西科学研究与技术开发计划课题 |
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| 摘 要： | 目的 探讨甘露醇对骨髓间充质干细胞(BMSCs)移植治疗血管性痴呆(VD)大鼠行为学及海马胆碱能系统活性的影响.方法 体外全骨髓培养结合细胞贴壁法培养大鼠BMSCs.采用双侧颈总动脉结扎法制备VD模型.将大鼠随机分为4组:(1)甘露醇预处理BMSCs组:造模4w后,先进行尾静脉注射20%甘露醇,10～30min后,再尾静脉注射BMSCs;(2)BMSCs组:造模4w后,经尾静脉注射等量BMSCs;(3)培养基组:造模4w后,经尾静脉注射等量BMSCs基础培养基;(4)假手术组:不进行任何干预.BMSCs移植4w后进行Morris水迷宫试验和大鼠海马内胆碱乙酰转移酶(ChAT)和乙酰胆碱酯酶(AChE)活性检测.结果 甘露醇预处理BMSCs组的ChAT和AChE活性均比BMSCs组、培养基组有明显提高(P<0.05),同时其行为学亦较BMSCs组、培养基组有明显改善(P<0.05).结论 甘露醇预处理后进行静脉注射移植BMSCs,使VD大鼠模型海马胆碱能系统的活性明显增强,并进一步改善VD大鼠的学习与记忆能力.
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| 关 键 词： | 甘露醇 骨髓间充质干细胞 血管性痴呆 胆碱乙酰转移酶 乙酰胆碱酯酶 |
The change of the ethology and cholinergic system of the hippocampus formation by intravenous administration of bone marrow mesenchymal stem cells with mannitol among vascular dementia rats |
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| ZHANG Tai-peng,Mo Xue-an,Liu Jin-ping,et al..The change of the ethology and cholinergic system of the hippocampus formation by intravenous administration of bone marrow mesenchymal stem cells with mannitol among vascular dementia rats[J].Journal of Apoplexy and Nervous Diseases,2011,28(4). |
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| Authors: | ZHANG Tai-peng Mo Xue-an Liu Jin-ping |
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| Institution: | ZHANG Tai-peng,Mo Xue-an,Liu Jin-ping,et al.(Department of Neurology,First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | Objective To explore the change of the ethology and cholinergic system of the hippocampus formation by intravenous administration of bone marrow mesenchymal stem cells(BMSCs)with mannitol among vascular dementia(VD) rats.Methods The BMSCs of rat were isolated and cultured by whole bone marrow adherence screening method.The models of VD rats were established through permanent ligation of bilateral common carotid arteries at an interval of 3 days for each artery.The healthy matured-male SD rats were divided r... |
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| Keywords: | Mannitol Bone marrow mesenchymal stem cells Vascular dementia ChAT AChE |
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