| 针刀松解法对膝骨关节炎大鼠中枢不同部位亮氨酸-脑啡肽的影响 |
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| 引用本文： | 郭长青,嵇波,陈幼楠,钟鼎文,金燕,刘清国,郭孟玮,张义,刘乃刚,陈占禄.针刀松解法对膝骨关节炎大鼠中枢不同部位亮氨酸-脑啡肽的影响[J].中国骨伤,2011,24(8):656-658. |
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| 作者姓名： | 郭长青 嵇波 陈幼楠 钟鼎文 金燕 刘清国 郭孟玮 张义 刘乃刚 陈占禄 |
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| 作者单位： | 北京中医药大学针灸推拿学院,北京,100029 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的：通过观察针刀松解法干预后膝骨关节炎（KOA）大鼠中枢不同部位亮氨酸-脑啡肽（L-ENK）的变化并与电针干预相比较,探讨针刀松解法在脊髓及脊髓以上水平的镇痛机制。方法：将60只健康SD大鼠随机分为空白组、模型组、电针组和针刀组。将4%木瓜蛋白酶溶液与0.3mol/L半胱氨酸溶液按1：1混合静置0.5h后分别于造模第1、4、7天注射于后3组大鼠左膝关节腔,每次20μl。造模第36天开始,相应给予针刀松解治疗或电针治疗,治疗3周后取大鼠腰膨大处脊髓及脑组织,分别测定脊髓、延髓、中脑、丘脑、海马的L-ENK含量。结果：造模后,模型组大鼠L-ENK含量在脊髓、中脑、海马较空白组差异均有统计学意义（P〈0.01）,延髓和丘脑L-ENK含量与空白组差异无统计学意义（P〉0.05）。针刀和电针干预后,针刀组海马中L-ENK含量较模型组升高,差异有统计学意义（P〈0.05）;针刀组中脑L-ENK含量较模型组降低,差异有统计学意义（P〈0.05）;海马中L-ENK含量针刀组较电针组高,差异有统计学意义（P〈0.05）;其余部位差异均无统计学意义（P〉0.05）。结论：针刀松解法对KOA大鼠中枢各水平的L-ENK有一定调节作用,其镇痛作用的发挥可能与其对中枢L-ENK的调节有关。
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| 关 键 词： | 骨关节炎 膝 电针 脑啡呔 亮氨酸 动物实验 |
| 收稿时间： | 9/3/2010 12:00:00 AM |
Affection of acupotomy lysis on Leu-Enkephalin(L-ENK) content in different parts of centrum of rats with knee osteoarthritis |
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| GUO Chang-qing,JI Bo,CHEN You-nan,ZHONG Ding-wen,JIN Yan,LIU Qing-guo,GUO Meng-wei,ZHANG Yi,LIU Nai-gang and CHEN Zhan-lu.Affection of acupotomy lysis on Leu-Enkephalin(L-ENK) content in different parts of centrum of rats with knee osteoarthritis[J].China Journal of Orthopaedics and Traumatology,2011,24(8):656-658. |
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| Authors: | GUO Chang-qing JI Bo CHEN You-nan ZHONG Ding-wen JIN Yan LIU Qing-guo GUO Meng-wei ZHANG Yi LIU Nai-gang and CHEN Zhan-lu |
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| Institution: | College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China;College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| Abstract: | Objective:To study the analgesia mechanism of needle-knife lysis in spinal cord and other parts of central nervous system by comparing the changes of Leu-Enkephalin(L-ENK) content in different parts of centrium of rats undergone needle-knife lysis and electro-acupuncture respectively.Methods:Sixty healthy SD rats were randomly devided into normal control group,model group,needle-knife lysis(NKL) group and electro-acupuncture(EA) group.4% papain solution mixed with 0.3 mol/L cysteine solutin in the ratio of ... |
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| Keywords: | Osteoarthritis knee Electroacupuncture Enkephalin leucine Animal experimentation |
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