| 高效液相色谱法测定马鞭草中熊果酸的含量 |
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| 引用本文： | 刘成红,刘云.高效液相色谱法测定马鞭草中熊果酸的含量[J].中国中药杂志,2002,27(12):916-918. |
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| 作者姓名： | 刘成红 刘云 |
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| 作者单位： | 北京总后卫生部药品仪器检验所中药室,北京,100071 |
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| 基金项目： | 国家药典委员会与法国进行的部分草药典合作交流资助项目 |
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| 摘 要： | 目的:建立马鞭草有效成分熊果酸的高效液相色谱分析方法,提高2000年版《中国药典》现有的马鞭草质量标准。方法:Kromasil C18色谱柱(4.6mm×250mm,5μm),柱温40℃,流动相甲醇-磷酸盐缓冲液(89:11),流速0.8 mL·min-1,检测波长210nm。结果:熊果酸在0.251-10.04μg呈良好线性关系,平均回收率98.1%,RSD为1.0%(n=6)。结论:本法优于2000年版《中国药典》采用的薄层扫描法,适用于马鞭草的质量控制。
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| 关 键 词： | 熊果酸 马鞭草 高效液相色谱法 |
| 文章编号： | 1001-5302(2002)12-0916-03 |
| 收稿时间： | 2001/12/21 0:00:00 |
| 修稿时间： | 2001年12月21 |
Derterminatiojl of Ursolic Acid in Herba of Verbena officinalis by HPLC |
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| LIU Cheng-hong;LIU Yun.Derterminatiojl of Ursolic Acid in Herba of Verbena officinalis by HPLC[J].China Journal of Chinese Materia Medica,2002,27(12):916-918. |
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| Authors: | LIU Cheng-hong;LIU Yun |
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| Institution: | Institute for Drug and Instrument Control of PLA, Beijing 100071, China. liuchenghong@chinaren.net |
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| Abstract: | OBJECTIVE: To establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis. METHOD: The column used was a Kromasil C18 (4.6 mm x 250 mm) packed with a 5 microns stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer monobasic sodium phosphate (MW = 119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1,000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0.8 mL per minute; the column was maintained at 40 degrees C; the DAD detector was set at 210 nm. RESULTS: The liner range was 0.251-10.04 micrograms (r = 1.0000). An average recovery of 98.1% (n = 6) was obtained with a RSD of 1.0%. CONCLUSION: The method is simple, accurate and suitable for the qualify control. |
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| Keywords: | ursolic acid European Verbena Herb HPLC |
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