阿魏酸、丹参多酚酸对持续性高正加速度引起大鼠颞肌波形蛋白表达的作用

目的 探索阿魏酸、丹参多酚酸对持续性高正加速度引起大鼠颞肌波形蛋白表达的作用. 方法 24只雄性Wistar大鼠随机分为8组,每组3只;第1组只在实验离心机上固定5 min作为对照组;第2组在+10 Gz条件下处置30 s、每日重复5次,每周4次,连续3周作为实验对照组;其余6组处置方法同实验对照组,且每日离心前30 min分别肌肉注射阿魏酸或丹参多酚酸(给药剂量分别为1、3、10 mg/kg),对照组及实验对照组给予等容积的生理盐水.用免疫组化染色方法观察每组波形蛋白的表达情况. 结果 对照组未发现波形蛋白的表达,而实验对照组波形蛋白染色呈现强阳性.不同剂量的阿魏酸、丹参多酚酸对于波形蛋白的表达呈现抑制作用,并且该抑制作用具有剂量依赖性. 结论 阿魏酸、丹参多酚酸对于持续性高正加速度引起的大鼠颞肌波形蛋白的表达有抑制作用,提示该类药物可有效地防治由于持续性高正加速度引起的颞颌关节疾病.

Effects of ferulic acid and depside salt from salvia miltiorrhiza on the expression of rat's temporal muscle vimentin induced by high sustained +Gz acceleration

Objective To observe the changes of vimentin expression of temporal muscles in rats that exposed to high sustained +Gz acceleration after administrating ferulic acid and depside salt from salvia mihiorrhiza. Methods Twenty-four male Wistar rats were randomly and averagely divided into 8 groups.Group 1 was control group and in where rats were only fixed on the resting centrifuge for 5 min without drug administration.Others were taken as test groups in where rats were exposed to +10 Gz environment for 30 s.The exposures for each test group were repeated 5 times a day,4 times a week and were tested for 3 weeks.In test groups,group 2 was taken as test control group where without drug administration while rats in other test groups were administrated either ferulic acid or depside salt from salvia mihiorrhiza 30 min prior to experiment with 3 different doses (1,3,10 mg/kg).Rats in control groups were taken 0.9% sodium chloride in same period with equivalent doses.Vimentin expression of temporal muscles was conducted by immunohistochemistry. Results Group 1 was not observed vimentin expression while group 2 showed significant positive.Taking different doses of ferulic acid and depside salt from salvia mihiorrhiza could inhibit expression of vimentin and such inhibition showed dose-dependent feature. Conclusions Ferulic acid and depside salt from salvia miltiorrhiza can inhibit vimentin expression of temporal muscle of rat that exposed in high sustained +Gz environment.It reveals that ferulic acid and depside salt from salvia miltiorrhiza may effectively prevent or cure +Gz induced temporomandibular joint disorder.