Survivin、bcl-2、caspase-3在牙源性角化囊肿中的表达及意义

目的检测凋亡相关蛋白survivin、bcl-2、caspase-3在角化囊肿中的表达,探讨其在角化囊肿发生发展中的意义。方法免疫组织化学方法检测20例角化囊肿(10例原发和10例复发)、10例含牙囊肿和10例根端囊肿中survivin、bcl-2、cas-pase-3的表达。结果Survivin、bcl-2、caspase-3阳性染色分别见于13(65%)、13(65%)、9(45%)例角化囊肿。Survivin、bcl-2在角化囊肿上皮基底层细胞中阳性表达,阳性率显著高于含牙囊肿和根端囊肿;caspase-3在角化囊肿上皮表层细胞表达。Survivin的表达与bcl-2正相关,与caspase-3无明显相关性。结论Survivin、bcl-2、caspase-3在角化囊肿形成和发展中起一定作用。

Expressions of survivin, bcl-2, caspase-3 and their significance in odontogenic keratocyst

Objective To investigate the expressions of survivin,bcl-2 and caspase-3 in human odontogenic keratocyst(OKC)and their possible roles in the cystogenesis and development of OKC.Methods Immunohistochemical assay was used to detect the expressions of survivin,bcl-2 and caspase-3 protein in 20 cases of OKC(including 10 primary OKC,10 recurrent OKC),and in 10 cases of dentigerous cyst,and 10 cases of radicular cyst.Results The expressions of survivin,bcl-2 and caspase-3 were 13(65%),13(65%),9(45%)cases of OKC,respectively.Survivin and bcl-2 positive stainings were mainly in the columnar or cuboidal cells of basal layer.There was a significantly higher positive ratio of OKC than those of dentigerous cyst and radicular cyst.Caspase-3 positive staining was mainly in surface layer flattened cells of OKC.The expression of survivin positively correlated with bcl-2 expression,but not with caspase-3 expression.Conclusion Survivin,bcl-2 and caspase-3 play an important role in generation and development of OKC.