急性脑缺血再灌注大鼠不同脑区乙酰胆碱酯酶活性的变化

本文旨在探讨胆碱能神经在脑缺血再灌注时的变化和意义。采用双侧颈总动脉夹闭（CCAO）的大鼠脑缺血动物模型，用改良的Ellman法测定大脑皮层、海马、间脑、纹状体和脑子五个脑区乙酰胆碱酯酶（AChE）活性，观察脑缺血30分钟和再灌注30分钟不同脑区AChE活性的变化。结果发现：在生理状态下不同胞区AChE活性不同，纹状体最高（181．8±1．7μ／g），皮层最低（24．1±0．4μ／g），CCAO30分钟皮层、海马、间脑、纹状体的AChE活性均明显下降，较正常大鼠分别降低278％、26．3％、16．9％、23．6％（P＜0．01），再灌注30分钟后分别回升18.4％、17.6％、10．2％、23．7％（P＜0．01）。而脑干在缺血和再灌注中无显著变化。结果提示不同胞区胆碱能活性程度不同；脑AChE活性对缺血和再灌注是敏感的，推测胆碱能神经可能在缺血性脑损伤中起重要作用。

Changes of acetylcholinesterase activities of various brain regions after acute cerebral ischemia and reperfusion in rat

The aim of this study was to investigate change and significance of cholinergic nerve after cerebral ischemia and reperfusion. This article applied improved Ellman method to determine acetylcholinesterase (AChE) activity of five brain regions (neocortex, hippocampus diencephalon striatum and brainstcm) induced by rat model of cerebral ischemia- common carotid artery occlusion (CCAO), we observed the changes of AChE activities of varions brain regions after ischemia 30 min and reperfusion 30 min. The results showed that AChE activities varied in various brain regions in physiological condition, the maximum in striatum was 181. 8±1. 7μ/g, whereas the minimum in neocortex was 24. 1± 0. 4μ/g. AChE activlties in neocortex, hippocampus diencephalon. striatum falled 27. 8%、26. 3%、16. 9%、23.6% respectively after CCAO 30 minutes. and raised 18. 4%,17. 6%,10. 2%,23. 7% respectively (P<0.01) after reperfusion 30 min. However, AChE activity of brainstem varied insignificantly after cerebral ischemia and reperfusion. It is suggested that cholinergic activities of various brain regions arc different, AChE is sensitlve to cerebral ischemia and reperfusion. It could infer that cholinegic nerve may play an important role in lsehemic brain injury.