| 三磷酸腺苷二钠HPLC检测的影响因素探讨 |
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| 引用本文： | 杨智华,邹斌,钱婷. 三磷酸腺苷二钠HPLC检测的影响因素探讨[J]. 中国医药工业杂志, 2002, 33(8): 403-404 |
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| 作者姓名： | 杨智华 邹斌 钱婷 |
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| 作者单位： | 广东肇庆星湖生物科技股份有限公司,广东肇庆,526040 |
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| 摘 要： | 用HPLC法检测三磷酸腺苷二钠（ATP）含量时,因成品中的二磷酸腺苷二钠（ADP）及蛋白质在259nm附近都有紫外吸收，因此ADP和蛋白质对含量测定有一定的影响。经试验对上述两种因素的影响进行了初步探讨。结显示当ADP的峰面积小于5％，蛋白质杂志峰总面积小于1％时，对HPLC法测定ATP含量无影响。
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| 关 键 词： | 三磷酸腺苷二钠 二磷酸腺苷二钠 蛋白质 HPLC 测定 |
| 文章编号： | 1001-8255(2002)08-0403-04 |
| 修稿时间： | 2001-08-07 |
Investigation of Determination of ATP by HPLC |
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| YANG Zhi Hua,ZOU Bin,QIAN Ting. Investigation of Determination of ATP by HPLC[J]. , 2002, 33(8): 403-404 |
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| Authors: | YANG Zhi Hua ZOU Bin QIAN Ting |
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| Abstract: | The content of adenosine disodium triphosphate (ATP) was determined by HPLC. The results were affected by adenosine disodium diphosphate (ADP) and protein which also had UV absorption at 259 nm. The effects of the two factors were discussed. When the area of ADP peak was less than 5%, and the total area of protein peaks were less than 1%, the results would not be influenced. |
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| Keywords: | ATP ADP protein HPLC determination |
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