IgG在人肝细胞肝癌中的表达及其临床意义

目的：研究肿瘤源性IgG在人肝细胞肝癌中的表达和分布情况及其与临床病理学指标的关系。方法：分别应用免疫组化二步法和原位杂交检测60例肝细胞肝癌组织及其周围正常肝组织中IgG蛋白和IgG mRNA的表达，分析IgG的表达水平与9项肝细胞肝癌的临床病理学指标之间的关系。结果：肝癌组织中IgG的表达明显高于其周围正常肝组织，且两者表达程度比较差异有统计学意义（P=0.001）；IgG在肿瘤组织中的异常高表达与肿瘤分化程度和pTNM分期呈负相关（r=-0.357，P=0.005；r=-0.296，P=0.021），与血浆AFP浓度呈正相关（r=0.336，P=0.009），与年龄、性别、肿瘤包膜的完整性、肿瘤大小、肿瘤个数和一年内是否复发或转移无相关性（P〉0.05）。结论：肝癌细胞所产生的IgG与肿瘤的分化、pTMN分期相关，可能作为辅助肝细胞肝癌病理学诊断和预后评估的一项重要指标。

IgG Expression in Hepatocellular Carcinoma and Its Significance

To investigate the expression and distribution of IgG in human liver cancer cells and its correlation with clinicopathological factors of hepatocellular carcinoma.Method：Fresh and paraffin-embedded tissues from 60 cases of HCC tumor with the matched normal liver specimens were detected by two-step immunohistochemistry and in situ hybridization for the expression of IgG protein and mRNA respectively. The relationship between cancer-derived IgG expression and 9 clinicopathological factors were analyzed statistically.Result：Signals of IgG protein in hepatocellular carcinoma cancer cells was significantly stronger than that in its corresponding normal tissues（P=0.001）;the over-expression of IgG was negatively correlated with several clinicopathological factors such as tumor differentiation grade and pTNM（r=-0.357,P=0.005;r=-0.296,P=0.021）,and positively associated with AFP concentration in serum （r=0.336,P=0.009）. However,no correlationship with age,gender,capsular infiltration,tumor size,tumor quantity and recurrence or metastasis of the disease within 1 year was found（P〉0.05）. Conclusion：Liver-cancer-derived IgG may has a profound impact on the differentiation and pTNM staging of liver cancer cell,and might be served as a tumour marker to help pathological diagnosis and evaluate the prognosis for patients.