| 人胎肝干细胞体外感染乙型肝炎病毒模型的建立 |
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| 引用本文： | 王姝,金敏,郭向飞,王新为,邱志刚,谌志强,李君文.人胎肝干细胞体外感染乙型肝炎病毒模型的建立[J].解放军预防医学杂志,2011,29(3):168-172. |
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| 作者姓名： | 王姝 金敏 郭向飞 王新为 邱志刚 谌志强 李君文 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050 |
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| 摘 要： | 目的 建立一种稳定的基于人胎肝干细胞的维持乙型肝炎病毒(HBV)复制和传代的体外细胞培养模型,为深入研究乙肝病毒的生命周期和发病机制提供必要的工具.方法 原代分离12～20周胎龄的胎肝干细胞,用乙肝病毒阳性血清感染胎肝干细胞;荧光定量PCR追踪检测细胞上清和细胞内乙肝病毒DNA及细胞内乙肝病毒复制中间体-闭合环状双链D...
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| 关 键 词： | 乙型肝炎病毒 胎肝干细胞 细胞培养 感染 复制 表达 |
IN VITRO INFECTION OF HUMAN FETAL HEPATIC STEM CELLS WITH HEPATITIS B VIRUS |
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| WANG Shu,JIN Min,GUO Xiang-fei,WANG Xin-wei,QIU Zhi-gang,SHEN Zhi-qiang,LI Jun-wen.IN VITRO INFECTION OF HUMAN FETAL HEPATIC STEM CELLS WITH HEPATITIS B VIRUS[J].Journal of Preventive Medicine of Chinese People's Liberation Army,2011,29(3):168-172. |
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| Authors: | WANG Shu JIN Min GUO Xiang-fei WANG Xin-wei QIU Zhi-gang SHEN Zhi-qiang LI Jun-wen |
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| Institution: | WANG Shu,JIN Min,GUO Xiang-fei,WANG Xin-wei,QIU Zhi-gang,SHEN Zhi-qiang,LI Jun-wen(Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China) |
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| Abstract: | Objective To establish a stable in vitro cell culture model to keep hepatitis B virus(HBV) replication and passage,in order to provide a useful tool for studying the life cycle of HBV and pathogenesis of HBV infection.Methods Human fetal hepatic stem cells were isolated from aborted fetuses of 12~20 weeks,and infected with sera from HBV-infected patients.Then HBV DNA,and cccDNA were detected by real-time PCR.Chemiluminescent method and immunohistochemical staining were used to examine HBsAg,HBeAg,HBcAg expr... |
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| Keywords: | hepatitis B virus human fetal hepatic stem cells cell culture infection replication expression |
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