玻璃化冷冻保存羊卵巢组织异种移植的实验研究

目的:探讨羊卵巢组织玻璃化冷冻复苏后异种移植的卵泡生长发育情况。方法:取绵羊的卵巢,将卵巢皮质切块,应用玻璃化快速冷冻法保存。羊卵巢组织片复苏后在裸鼠的颈部皮下移植,每只裸鼠移植2块组织,于移植1个月后获取移植物,HE染色观察存活卵泡的情况。结果:共移植组织16块,取材时肉眼可见其中一块组织纤维化萎缩,镜下见此块组织纤维化,移植组织存活率87.5%(14/16)。HE染色观察每只移植小鼠的2块移植物中基本只有1块有可视卵泡。1只鼠未见可视卵泡;1只鼠见1个不典型原始卵泡;1只鼠有2个不典型原始卵泡;余下5只鼠的移植卵巢组织中均可见多个典型的存活卵泡。结论:羊卵巢组织经冷冻复苏后能够成功移植于裸鼠的颈部皮下,大部分卵泡处于良好的存活状态。

Experimental study on xenotransplantation of sheep ovarian tissue cryopreserved by vitrification

Objective： To investigate the growth and development condition of follicles in sheep＇s ovarian tissue after cryopreservation and recovery.Methods： The ovarian cortex cuts of sheep were rapidly cryopreserved by vitrification and transplanted under the cervical subcutaneous of nude mice after recovery.The grifts were obtained one month later and HE staining was exerted to observe the survival follicles.Results： There was one grift fibrosis and atrophy visible by naked eyes.Another grift was fibrosis in microscopic observation.There was 14 grifts surviving in the total 16 grifts and the survival rate was up to 87.5%.The follicles were almost visible in one of the two grifts in one mouse.There was no visible follicle in the mouse No.8 and there was respectively one and two atypical primordial follicles in the mice No.2 and No.6.Many typical survival follicles could be seen in all the grifts of the left five mice.Conclusion： The ovarian tissue of sheep could survive when transplanted under the cervical subcutaneous of nude mice after cryopreservation and recovery and the follicles were in good growth and development condition.