| GnRHa长方案超促排卵对围着床期小鼠子宫内膜溶血磷脂酸受体3(LPAR3)蛋白的表达及胞饮突发育的影响 |
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| 引用本文： | 郭燕红,张雷,邵素霞,赵春芳,龚淼.GnRHa长方案超促排卵对围着床期小鼠子宫内膜溶血磷脂酸受体3(LPAR3)蛋白的表达及胞饮突发育的影响[J].生殖与避孕,2011,31(5):289-293. |
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| 作者姓名： | 郭燕红 张雷 邵素霞 赵春芳 龚淼 |
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| 作者单位： | 1. 厦门大学附属第一医院生殖医学科
2. 河北医科大学组织学与胚胎学教研室,石家庄,050011 |
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| 摘 要： | 目的:研究GnRHa/hMG/hCG控制性超促排卵对围着床期小鼠子宫内膜溶血磷脂酸受体3(LPAR3)蛋白的表达及胞饮突发育的影响。方法:雌性未孕昆明系小鼠72只,随机分为控制性超促排卵组(COH)和自然受孕组(NC),再按交配成功后天数每组分成4个亚组,接受相应的建模处理。用Western blotting检测性交后3~6 d(孕3~6 d)小鼠子宫内膜LPAR3蛋白的表达;同时用扫描电子显微镜观察子宫内膜胞饮突的发育。结果:COH组性交后3 d亚组LPAR3蛋白表达最强,NC组4 d亚组表达最强,但两者表达量无显著差异。COH组3~5 d亚组胞饮突发育不同步,大小不一,胞饮突数量较NC组相应时间明显减少,呈局灶性分布。NC组3 d亚组多为发育中的胞饮突,4 d亚组几乎均为完全发育的胞饮突,5 d亚组多为退化的胞饮突。结论:GnRHa控制性超促排卵可能通过改变小鼠子宫内膜LPAR3蛋白和超微结构的时序变化来影响子宫内膜容受性,从而影响胚泡着床。
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| 关 键 词： | 控制性超促排卵(COH) 溶血磷脂酸受体3(LPAR3) 胞饮突 子宫内膜容受性 |
Effect of Long Protocol Controlled ovarian hyperstimulation by GnRHa on the expression of LPAR3 protein and the development of pinopodes in mouse uterine endometrium during periimplantation Period |
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| Yan-hong GUO,Lei ZHANG,Su-xia SHAO,Chun-fang ZHAO,Miao GONG.Effect of Long Protocol Controlled ovarian hyperstimulation by GnRHa on the expression of LPAR3 protein and the development of pinopodes in mouse uterine endometrium during periimplantation Period[J].Reproduction and Contraception,2011,31(5):289-293. |
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| Authors: | Yan-hong GUO Lei ZHANG Su-xia SHAO Chun-fang ZHAO Miao GONG |
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| Institution: | Yan-hong GUO,Lei ZHANG,Su-xia SHAO,Chun-fang ZHAO,Miao GONG(Department of Histology and Embryology,Hebei Medical University,Shijiazhuang,050011) |
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| Abstract: | Objective: To investigate the influence of controlled ovarian hyperstimulation(COH) by GnRHa/hMG/hCG long protocol treatment to the expression of lysophosphatidic acid receptor 3(LPAR3) protein and the development of pinopodes.Methods: Healthy female unpregnant Kunming mice were randomly divided into COH group and natural conception group(NC group) and ferther divided into 4 subgroup(3-6 d post coitus,per sub-group was 6 mice).The expression of LPAR3 protein in uterine endometrium tissue was detected by Wes... |
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| Keywords: | controlled ovarian hyperstimulation(COH) lysophosphatidic acid receptor 3(LPAR3) pinopodes endometrial receptibility |
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