甲状腺乳头状癌中Kiss-1、Ki-67、VEGF-C的表达及其定量相关性研究

目的：探讨Kiss-1、Ki-67和VEGF-C在甲状腺乳头状癌（ papillary thyroid carcinoma ，PTC）和甲状腺滤泡性腺瘤（ follicular adenoma ， FA）中的表达及意义。方法采用免疫组织化学染色方法和显微图像定量分析技术测定44例PTC及12例FA石蜡包埋组织中Kiss-1、Ki-67及VEGF-C的表达。结果 PTC组Kiss-1和VEGF-C的积分光密度分别为475．56±126．02和805．29±226．05，Ki-67蛋白的增殖指数为（3．36±1．11）％，与FA组相比差异显著（P＜0．05）。有淋巴结转移的PTC组Kiss-1和VEGF-C的积分光密度分别为408．12±124．05和912．63±108．12，Ki-67蛋白的增殖指数为（3．93±0．92）％，与无淋巴结转移的PTC组相比差异显著（P＜0．05）。有被膜浸润的PTC组Kiss-1和VEGF-C的积分光密度分别为425．58±87．38和891．37±149．36， Ki-67蛋白的增殖指数为（3．79±1．09）％，与无被包膜浸润的PTC组相比差异显著（P＜0．05）。线性相关分析显示，Ki-67与VEGF-C在PTC和FA组的表达呈正相关（P＜0．05）；Kiss-1与Ki-67、VEGF-C在PTC和FA组的表达呈负相关（P＜0．05）。结论 Kiss-1、Ki-67和VEGF-C有助于PTC与FA的鉴别诊断，并可作为PTC患者预后判断的指标。

Expression of Kiss-1, Ki-67 and VEGF-C in papillary thyroid carcinoma and its stereological determination

Objective To explore the expression and significance of Kiss-1, Ki-67 and VEGF-C in papillary thyroid carcinoma（PTC） and thyroid follicular adenoma （FA）.Methods Forty-four cases of PTC and twelve cases of FA paraffin-embedded tissues were used .Immunohistochemical staining and microscopic image analysis technique were used to analyze the expression of Kiss-1, Ki-67 and VEGF-C.Results The integrated optical density （IOD） of Kiss-1, and VEGF-C in the PTC groups was 475.56 ±126.02 and 805.29 ±226.05,respectively.The proliferation index of Ki-67 protein was （3.36 ±1.11） %and the difference between the PTC and FA groups was statistically significant （P〈0.05）.The IOD of the above two indices was 408.12 ±124.05 and 912.63 ±108.12 in the PTC with lymph node metastasis group , respectively, while the proliferation index of Ki -67 protein was （3.93 ±0.92） % and the difference vs the group without lymph node metastasis was significant （ P 〈0.05 ） .In the PTC with capsular infiltration group the IOD of above two was 425.58 ±87.38 and 891.37 ±149.36, the proliferation index of Ki -67 protein was （3.79 ±1.09） %and the difference with PTC group without capsular infiltrtion was statistically significant （P〈0.05）.Linear correlation analysis showed that Ki-67 and VEGF-C were with positively correlated in PTC and FA tissues （P〈0.05）,while Kiss-1 and Ki-67, VEGF-C were with negatively correlated in PTC and FA tissues （P〈0.05）.Conclusion Kiss-1, Ki-67 and VEGF-C can facilitate the differential diagnosis of PTC and FA , serving as prognostic indicators in patients with PTC .