神经干细胞与骨髓间充质干细胞移植对脊髓损伤修复的比较

目的:比较神经干细胞和骨髓间充质干细胞移植治疗脊髓损伤效果与机制的差异。方法:用成年Wist-ar大鼠建立脊髓半横切模型,随机分为神经干细胞(NSCs)组(n=10)、骨髓间充质干细胞(BMSC)注射组(n=10)、PBS注射组(n=10)及只打开椎板的假手术组(n=10)。移植后行BBB运动功能学评分,半切位置的免疫组化及核磁成像比较。结果:BBB评分NSCs注射组明显高于BMSCs注射组,NSCs注射组和BMSCs注射组均明显高于PBS注射组,脊髓切片中可观察到被标记的NSCs及BMSCs,核磁成像显示移植后半切形成的脊髓空洞有所减小。结论:静脉注射NSCs、BMSCs均能改善脊髓损伤大鼠的运动功能,但注射NSCs效果更明显。静脉注射干细胞后,细胞可迁移至脊髓损伤的位置,核磁成像显示脊髓空洞有所减小,提示干细胞可能通过补充或替代损失的神经细胞,修复已缺失的神经组织和功能性神经单位,重建神经环路。

Comparison of Neural Stem Cells and Bone Marrow Mesenchymal Stem Cells Transplantation for Spinal Cord Injury

Objective:To compare the curative effect of transplantation between the NSCs and BMSCs for spinal cord injury.Methods:Spinal cord semi-intersection Wistar rat models were established and were randomly divided into groups of BMSC injection(n = 10),NSCs injection(w = 10),PBS injection(n = 10),and sham operation group of only the lamina opened(n = 10).After the transplantation, the BBB locomotor function scoring,immunohistochemistry and magnetic resonance imaging of position half-cut were adopted for evaluation.Results:Scores of BBB of NSCs injected group was significantly higher than BMSCs injected group,scores of BBB of NSCs injected group and BMSCs injected group were significantly higher than that of PBS injected group.Labeled NSCs and BMSCs can be observed in slices of half cut part of spinal cord.The MRI resonance imaging showed that after transplantation,syringomyelia has been reduced.Conclusion:Intravenous injection of NSCs and BMSCs can improve the motor function of spinal cord injury in rats, but the effect of injection of NSCs was better.After the intravenous injection of stem cells,the cells can migrate into the location of the spinal cord injury,magnetic resonance imaging showed syringomyelia has been reduced,and it suggests that stem cells may supplement or replace lost nerve cells,repair the deficient neural organization and the functional neural units and rebuild nerve loops.