氢气对吸入性损伤大鼠急性肺损伤的保护作用及量效研究

目的：探讨不同剂量氢气饱和生理盐水（HS）对烟雾吸入性损伤大鼠急性肺损伤的保护作用.方法：30只SD大鼠随机分为5组：对照组（C）、吸入性损伤组（Ⅰ）、氢气饱和生理盐水低剂量组（2.5 mL/kg,HSL）、中剂量组（5mL/kg,HSM）、高剂量组（10 mL/kg,HSH）,24 h后留取标本,透射电镜观察肺组织病理变化,检测血清中肿瘤坏死因子α（TNF-α）,肺组织匀浆的丙二醛（MDA）含量,免疫组织化学法测定肺组织核因子-κBp65（NF-κBp65）的表达,TUNEL检测肺组织凋亡情况.结果：中、高剂量氢气饱和生理盐水能明显减轻吸入性损伤大鼠的急性肺损伤,降低炎性因子（TNF-α）的释放,减轻组织氧化损伤（MDA低于致伤组）,抑制肺组织NF-κBp65的活化及细胞凋亡,且HSH优于HSM,HSM、HSH组间差异有统计学意义（P＜0.05）,而HSL组效果不明显.结论：氢气饱和生理盐水可减轻吸入性损伤大鼠的急性肺损伤,且存在一定的量效关系.

Protective effects of hydrogen gas on acute lung injury induced by smoke inhalation in rats and its doseeffect relationship

Objective：To discuss the therapeutical effect on smoke inhalation injury in rats under different doses of hydrogen-rich saline.Methods：Thirty rats were randomly divided into 5 groups：control group （C）,inhalation group （Ⅰ）,low dose group （2.5 mL/kg,HSL）,medium dose group （5 mL/kg,HSM）,and high dose group （10 mL/kg,HSH）.At 24 hours after injured,all rats were sacrificed and specimens were taken.The histopathological changes of lung tissue were observed using electron micrography.Tumor necrosis factor-α （TNF-α） in plasma was quantified,malondialdehyde （MDA） content of lung tissue was also detected,the NF-κBp65 in lung speciments was examined by immunohistochemistry technology,and the apoptosis of lung was monitored by terminal deoxynucleotidyl transfercase dUTP nik end labeling （TUNEL） assay.Results：When a comparison was made with the inhalation group,hydrogen-rich saline treatment could significantly relieve acute lung injury induced by inhalation injury as indicated by ultrastructural histopathologic changes under electron micrography and decrease TNF-α level.Furthermore,hydrogen-rich saline could inhibit lipid oxidation （MDA level was significantly decreased）,the expression of NF-κBp65 and apoptosis of lung tissue,especially in medium and high dose group,while this change was not significant in low dose group.Conclusion：Hydrogen-rich saline treatment could significantly relieve acute lung injury induced by inhalation injury,with dose-effect relationship.