养阴抗毒散对X线照射小鼠T细胞亚群及红细胞黏附功能的影响

目的: 研究小鼠急性中度骨髓型放射损伤细胞免疫功能的变化及养阴抗毒散的防护作用.方法: 以6 Gy X射线照射小鼠为动物模型,随机分为正常对照组（A）、照射组（B）及照射加中药组（C）.用流式细胞仪直接免疫荧光染色法测定外周血T淋巴细胞亚群,酵母菌红细胞花环试验法测定红细胞免疫能力.结果: 与A组比较,B,C两组小鼠CD4,CD8百分率显著减少(P<0.01)；B组CD4/CD8比值明显降低(P<0.01).B组小鼠RBC-C3bR花环率第12,20,28日降低(P<0.01),RBC-IC花环率第4日和第8日升高(P<0.01)；C组小鼠RBC-C3bR花环率仅在第12日下降(P<0.01),RBC-IC花环率仅在第4日升高(P<0.01)；C组RBC-C3bR花环率在第4,12,20,28日均高于B组(P<0.01).结论:6 Gy X线照射可造成小鼠细胞免疫功能低下,养阴抗毒散对此有一定的防治作用.

Effects of Yangyin Kangdu powder on T lymphocyte subpopulations and erythrocyte immune functions in irradiated mice

AIM: To study the changes of cellular immune function in mice with moderate acute irradiation injuries and the protective effects of Yangyin Kangdu powder, a traditional Chinese medicine, on the injuries. METHODS: The mice models of 6 Gy X ray irradiation were established and the mice were randomized into control (A), irradiation (B) and irradiation plus Yangyin Kangdu powder (C) groups. T lymphocyte subpopulations of peripheral blood (including CD4 and CD8) were measured by direct microvolume whole blood immunoflurescent staining technique by flow cytometry, and erythrocyte immune functions (including RBC C 3bRR and RBC ICR) and WBC quantity were observed to evaluate the immune function of mice. RESULTS: The values of CD4, CD8 in groups B and C were significantly lower than those in control group ( P < 0.01) and the CD4/CD8 ratio in group B was significantly lower than that in control group ( P <0.01). The RBC C 3bRR ratio in group B significantly decreased on d 12, 20 and 28 after irradiation ( P <0.01) and the RBC ICR rosette ratio increased on d 4, 8 and subsequently declined to normal range. The RBC C 3bRR rosette ratio decreased only on the 12th d and the RBC ICR rosette ratio increased only on the 4th d in group C. The RBC C 3bRR rosette ratio in group C was higher than that in group B on d 4, 12, 20 and 28 ( P <0.01). CONCLUSION: The cellular immune function of the mice irradiated by 6 Gy X ray is low and Yangyin Kangdu powder has some protective effects on the cellular immune function after irradiation injuries.