款冬花散对支气管扩张症痰热蕴肺证模型大鼠气道炎症的影响

目的:观察款冬花散对支气管扩张症痰热蕴肺证模型大鼠气道炎症的影响.方法:70只大鼠随机分为空白组、款冬花散自身对照组、模型组、罗红霉素组、款冬花散干预组(低、中、高剂量),每组10只.采用绿脓杆菌液气管注入制备支气管扩张症动物模型.采用款冬花散干预2周后取材,观察肺组织形态改变、肺泡灌洗液(BALF)中白三烯B4(LT...

Effect of Kuandonghua formula on airway inflammation in rats for bronchiectasis with the phlegm-heat stagnation syndrome

Objective:To observe the effect of Kuandonghua formula on airway inflammation in rats for bronchiectasis with the phlegm-heat stagnation syndrome.Methods:A total of 70 rats were randomly divided into the blank group,the self-control group,the model group,the roxithromycin group,the Kuandonghua formula treatment group at low,middle and high dosage with 10 rats in each group.The rat model was induced by intratracheal injection of pseudomonas aeruginosa dilution.Rats in the treatment group were treated with Kuandonghua formula for 2 weeks.The morphological changes of lung tissue,concentrations in bronchoalveolar lavage fluid(BALF)and expressions of mRNA in the lung tissue including LTB4,TNF-α,IL-8 and IL-10 were evaluated.Results:Compared with the blank group,the inflammatory infiltration of lung tissue,concentrations in BALF and expressions of mRNA in the lung tissue including LTB4,TNF-α,IL-8 and IL-10 of the model group were significantly increased(P<0.01).Compared with the model group,the inflammatory infiltration of lung tissue,concentrations in BALF and expressions of mRNA in the lung tissue with regard to LTB4,TNF-α,IL-8 and IL-10 of the Kuandonghua formula treatment group were significantly decreased,which the effect of high dosage was superior to low and middle dosage(P<0.01).Conclusion:Kuandonghua formula can reduce airway inflammation in rats for bronchiectasis with the phlegm-heat stagnation syndrome,and it presents a certain quantity-effect relationship trend.