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Isolation and purification of influenza virus mRNA coding for M protein
Authors:A Mikhejeva  S Melnikov  V Ginzburg  Y Ghendon
Affiliation:Moscow Research Institute of Virus Preparations, Moscow USSR
Abstract:The mRNA coding for membrane protein (M protein) of influenza virus has been isolated by means of double immunoprecipitation of polysomes prepared from influenza virus-infected cells. The size of the mRNA (13 S) and its messenger activity in a wheat germ cell-free system indicate the lack of contamination with other mRNAs or ribosomal RNAs. Hybridization analysis has demonstrated complementarity of the isolated mRNA to the virion RNA.
Keywords:To whom requests for reprints should be sent.
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