iNOS,FⅧ-RAg在喉鳞状细胞癌的表达与血管形成的关系

目的探讨诱导型一氧化氮合酶(iNOS)和FⅧ-RAg在喉癌组织中的表达及其侵袭转移过程中的作用。方法采用免疫组化Elivi-sion法检测30例喉鳞状细胞癌、10例声带息肉中iNOS和FⅧ-RAg的表达,并检测微血管密度(MVD)。结果30例喉癌组织中iNOS的阳性表达率为80%(24/30),声带息肉中iNOS的阳性表达率为10%(1/10)。FⅧ-RAg在喉癌组织和声带息肉的阳性表达率分别为70%(21/30),0%(0/10)。FⅧ-RAg,iNOS阳性表达两组间差异有统计学意义,P<0.025。喉癌组织和声带息肉中MVD分别为65.85±13.06,5.64±3.57,两组间差异有统计学意义(P<0.001)。结论iNOS,FⅧ-RAg在喉癌组织中的高表达,提示iNOS,FⅧ-RAg可促进血管形成,可能与喉癌的发生、发展和侵袭转移有关。因此,选择性抑制iNOS和FⅧ-RAg可减少肿瘤的发展和侵袭转移。

Relation between Expression of Inducible Nitric Oxide Synthase and FⅧ Related Antigen and Angiogensis in Laryngeal Carcinoma

Objective To investigate the expressions of inducible nitric oxide synthase (iNOS) and FⅧ related antigen (FⅧ-Rag) and their role in the occurrence and the development of laryngeal carcinoma. Methods Immunohistochemical method (Elivision) was used to examine the expressions of iNOS and FⅧ-RAg in 30 cases of laryngeal carcinoma and 10 cases of vocal cord polyp, and micro-vascular density (MVD) was measured as well. Results The positive rates of iNOS were 80.0% and 10% and FⅧ-RAg was 70% and 0 in the laryngeal carcinoma group and in the vocal cord polyp group, respectively. There were significant differiences in the expressions of iNOS, FⅧ-RAg and MVD between the two groups (P<0.05, P<0.001). Conclusion There is high iNOS and FⅧ-RAg expressions in laryngeal carcinoma which indicates that there is a new agniogensis and that iNOS and FⅧ-RAg plays an important role in the incidence, development and metastasis of laryngeal carcinoma. Specific iNOS and FⅧ-RAg inhibitor may reduce the development and the metastasis of laryngeal carcinoma.