人鼻咽不同部位粘膜上皮PCNA表达的比较研究

目的 探讨鼻咽部肿瘤发生位置差异性的机理。方法 应用免疫组织化学与图像分析技术对人鼻咽粘膜上皮增殖细胞核抗原（ＰＣＮＡ）的表达状况进行了比较研究。结果 鼻咽上皮的ＰＣＮＡ标记指数以及ＰＣＮＡ阳性细胞的分布状况均随发育时期及其所处鼻咽部位置的不同而变化，成人鼻咽的顶壁和侧壁粘膜上皮具有相对较高的ＰＣＮＡ标记指数，与人鼻咽癌的易感部位正好形成对应关系。结论 鼻咽部不同部位的粘膜上皮在上皮细胞群体增殖活

COMPARATIVE STUDIES OF PCNA EXPRESSION AMONG MUCOSAL EPITHELIA IN DIFFERENT REGIONS OF HUMAN NASOPHARYNX

Objective In order to investigate the relationship between the expression regulation of Cx genes and cellular differentiation in various organs during embryonic development.Methods Using connexin genes Cx26,Cx31,Cx31 1,Cx32,Cx37,Cx40,Cx43 and Cx46 as the molecular probes,the Northern blot hybrydization was done,we studied the expression level of Cx genes in different stages of embryonic organs.Results The results demonstrated that:1 Cx31,Cx31 1 and Cx46 don't express while other Cx genes express in the liver,stomach,lung and kidney of different stages of embryonic organs.2 The expression of Cx genes show different level in various organs at different development stages of embryogenesis.3 The expression of Cx genes shows the organic diversity,for instance,the expression of Cx32 in liver,Cx37 in stomach,Cx40 in lung and Cx43 in kidney of embryonic organs.4 The expression of Cx genes is overlapping in various organs.In above organs,Cx26 presents different states of expression .5 The expression level of Cx genes is unanimous with cellular differentiation.Conclusion Cx gene might be a key condidate gene to regulate some differentiational events associated with cellular differentiation,proliferation,morphogenesis in early embryo.