应用单宁酸增加致密核心小泡电子密度的研究

对如何应用单宁酸提高密核心小泡电子密度进行了研究，结果发现实验组动物应用单宁酸后，其延髓背角胶状质的超微结构出现了如下特征性变化；（１）膜结构清晰度增加，反差鲜明；（２）大、小致密核心小泡均被媒染，其电子密度显著提高、大致密核心小泡于突触部位胞吐入细胞间隙内的介质，被即时媒染、固定。

THE APPLICATION OF TANNIC ACID TO INCREASE ELECTRON DENSITY OF DENSE CORED VESICLES

We used the tannic acid(TA) to increase the electron density of dense cored vesicles. The results showed that the ultrastructure of the substantia gelatinosa in medullary dorsal horn changed characteristically after the application of TA to the experimental animals. (1) The structure of plasmalemma was more clearly visulised. (2) Both large and small dense cored vesicles were well mordantly stained by TA, their electron density increased significantly, and the transmitters were stained and fixated promptly as they were released from large dense cored vesicles into intercellular space by exocytosis at the nonsynaptic sites. The experiment also showed that the effect was not obvious when the concentration of perfusion solution containing TA was below 0.25%, but when it was above 0.5% the normal structures were destinctly resolutive and disperse. We found that the ideal result could be obtained by perfusing the animals first with 0.25% TA normal saline solution slowly and then with 0.5% TA double aldehyde solution quickly through the aorta.