幽门螺杆菌和p53基因与胃癌的关系研究

研究幽门螺杆菌（ｈｅｌｉｃｏｂａｃｔｅｒ ｐｙｌｏｒｉ）感染、突变型 ｐ５３基因表达与胃癌的关系，以及胃癌时 ＨＰ感染与突变型ｐ５３基因表达的关系。方法：用快速尿素酶试验和ＥＬＩＳＡ法检测ＨＰ和ＨＰ抗体，用免疫组化方法测定突变型ｐ５３蛋白。结果：胃癌和胃癌前病变组ＨＰ阳性率高于其它组（Ｐ＜０．０５），而胃癌组ＨＰ阳性率和胃癌前病变组无显著差异（Ｐ＞０．０５），胃癌组突变型 ｐ５３蛋白阳性率高于胃癌前病变组（Ｐ＜０．０５），胃癌中 ＨＰ阳性者突变型 ｐ５３蛋白阳性率高于 ＨＰ阴性者（Ｐ＜０．０５）。结论：胃癌的发生和ＨＰ感染、突变型ｐ５３基因表达有关，胃癌时ＨＰ感染与突变型ｐ５３基因表达也存在相关性。

RELATION BETWEEN HELICOBACTER PYLORI, EXPRESSION OF p53 AND GASTRIC CANCER

Objective: To investigate the relation ship between helicobacter pylon(HP), mutant p53 expression in gastric cancer. Methods: Using immediate urease test and ELISA to detect HP, immuno histochemical staining to detect mutan p53 protein. Results: The positive rate of HP in gastric cancer and gasric precancerous conditions is higher than that of the other (P<0.05), but comparing gastric cancer with precancerous conditions, the two positive rates of HP have no significant difference (P>0.05). The positive rate of mutant p53 protein in gastric cancer is higher than in precancerous conditions (P<0.05) HP positive gastric cancer has a higher positive rate of mutan p53 protein than HP negative gastric cancer (p<0.05). Conclusion: HP inflammation and mutant p53 expression have a role in the development of gastric cancer. In gastric cancer HP inflammation has a connection with the expression of mutant p53 gene.