| Abstract: | Objective To optimize the extraction technology of Taxus x media by using the contents of Paclitaxel and 10-deacetylbaccatin (10-DAB), two representative active diterpene alkaloids of taxane type from T. x media, as evaluation standard. Methods The smashing tissue extraction (STE) of Paclitaxel and 10-DAB from T. x media, was investigated by comparing with ultrasonic extraction (UE) which was one of the modern technologies of extraction. Results STE was more efficient than UE, and the contents of 10-DAB and Paclitaxel in the extracts obtained by STE were higher than those by UE. Conclusion STE is a fast, high-performance, and energy-saving technology for the extraction of diterpene alkaloids of taxane type. STE also provides a simple, component-safe, workable, and highly efficient method for the extraction of active natural product. |
