丙型肝炎患者外周血单个核细胞HCV感染的电镜研究

目的 以常规电镜和免疫电镜技术，发现和证实慢性丙型肝炎患者外周血单个核细胞９ＰＢＭＣｓ）内丙型肝炎病毒（ＨＣＶ）颗粒。试图在病毒形态学和形态发生学上证实ＰＢＭＣｓ的ＨＣＶ感染和复制。方法 以逆转录多聚酶链反应（ＲＴ－ＰＣＲ）和免疫组织化学方法，分别检测２８例患者ＰＢＭＣｓ内ＨＣＶ ＲＮＡ和ＨＣＶＡｇ，对其中阳性标本重点进行电镜研究。结果 ＨＣＶ ＲＮＡ和ＨＣＶ Ａｇ阳性检出率分别为７７．２７％（１

Identificationand visualzation of virus-like particles in peripheral blood mononuclear cells (PBMCs) ofpatients with chronic hepatitis C

Objective PBMCs from patients with chronic hepatitis C were examined by electron microscopy(EM), immune EM(IEM) combined with immunohistochemistry to trace the infection and morphology of HCV in the PBMCs. Methods The PBMCs from 28 patients with chronic hepatitis C were analyzed firstly for HCV RNA and HCV antigens by RT PCR and immunohistochemistry respectively. The PBMCs with positive HCV RNA and HCV antigens were then observed by electron microscopy. Results The positive rate of HCV RNA and HCV antigens were 77 27%(17/22) and 75 00%(21/28) respectively. Two types of polymorphic HCV like particles with diameter of approximately 65 nm and 110 nm were observed in cytoplasmic vesicles of the PBMCs from HCV Ag positive patients. 10 patients with HCV Ag over expression were studied by electron microscopy and immune election microscopy. The budding phenomenon of the particles could be also visualized in the cytoplasmic vesicles of the PBMCs. Some ultrastructural changes showed an increased ratio of cytoplasm to nucleus and pyknosis of nucleus was also suggestive of the virus infection. Conclusion The EM study demonstrated the infection and replication of HCV in the PBMCs of chronic hepatitis C patients by morphology and morphogenesis.