桔梗总皂苷体外抗解脲脲原体作用实验研究

目的：通过MIC、MBC、TIME-KILL曲线及快速杀菌测定，观察PGS在体外对Uu生长是否具有抑制作用。方法：采用倍比稀释法测定MIC和MBC，利用定量PCR检测不同浓度药物对Uu生长的抑制和快速杀灭作用。结果：PGS体外抗Uu的MIC为16-32μg•mL-1；MBC为32-64μg•mL-1。16和32μg•mL-1的 PGS对Uu的繁殖具有一定的抑制作用，当浓度达到64μg•mL-1时可完全快速抑制Uu的生长繁殖。结论：PGS在体外具有抑制Uu生长繁殖作用。

Study on anti-ureaplasma urealyticum activity of platycodon grandiflorum saponins in vitro

Objective: To observe the activity of Platycodon grandiflorum saponin (PGS) against Ureaplasma Urealyticum(Uu) through experiments of MIC, MBC , TIME-KILL and rapid bactericidal action. Method: MICs and MBCs were determined by broth microdilution method. The concentration of Uu was detected by quantitative PCR after diferent incubate time. Result: MICs were 16-32μg•mL-1 while MBCs were 32-64μg•mL-1 for PGS against Uu in vitro. Replication of Uu in PPLO was inhibited  by PGS when the concentration was between 16 and 32μg•mL-1. The number of Uu in PPLO broth was reduced and the growth was completely inhibited when the concentration of PGS reached 64μg•mL-1. The growth of Uu was inhibited after mixed with PGS for 1min in the concentration of 64μg•mL-1. Conclusion: PGS has anti-Ureaplasma Urealyticum activity in vitro, and could inhibite the growth of Uu rapidly.