胃癌亚甲基四氢叶酸还原酶基因 1298AC多态性与H.pylori感染的关系

目的　探讨重庆地区胃癌人群亚甲基四氢叶酸还原酶 (MTHFR)基因 12 98AC多态性及与H .pylori感染的关系。方法　采用聚合酶链反应和限制性片段长度多态性 (PCR RFLP)技术 ,检测 12 2例胃癌和 10 1例正常对照的MTHFR基因 12 98AC多态性 :采用PCR和Warthin Starry银染色法检测幽门螺杆菌 (H .pylori)。结果　胃癌组 12 98AA、12 98AC和12 98CC 3种基因型频率分别为 5 9.8%、3 6.1%和 4.1% ,与对照组中 3种基因型频率 5 7.4%、3 7.6%和 5 .0 %相比无显著差异 (P >0 0 5 )。以 12 98AA基因型的OR为 1.0 0 ,胃窦癌AC基因型的OR为 0 .87( 95 %CI,0 .42～ 1.82 ) ,CC基因型的OR为0 .41( 95 %CI ,0 .0 5～ 3 .72 )。胃癌MTHFR 12 98AC 3种基因型中H .pylori的检出率无明显差别 (P >0 0 5 )。结论　重庆地区人群中MTHFR 12 98AC多态性可能是胃窦癌的保护因素 ,MTHFR 12 98AC多态性与H .pylori感染无关。

Relationship between polymorphisms in methylenetetrahydrofolate reductase gene 1298AC and H.pylori infection in gastric cancer

Objective To explore the relationship between the polymorphisms in methylenetetrahydrofolate reductase (MTHFR) gene 1298AC and Helicobacter pylori (H. pylori) infection in patients with gastric cancer from Chongqing. Methods The polymorphisms in MTHFR gene A1298C were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and H. pylori was detected by PCR and Warthin-Starry silver stain methods. Results The polymorphisms in MTHFR 1298AC in 122 cases of gastric cancer and 101 cases of normal controls were analyzed. The genotypic frequencies of MTHFR 1298AA, 1298AC, and 1298CC were 59.8%, 36.1%, and 4.1% in gastric cancer, but 57.4%, 7.6%, and 5.0% in the controls, respectively. There was no significant difference in distribution of MTHFR 1298 A C genotype between gastric cancer and controls (P>0.05). The individuals with 1298CC genotype had a 0.41-fold (95%CI, 0.05-3.72) reduced risk of developing gastric cancer antrum as compared with those who had 1298AA genotype. The polymorphisms in MTHFR 1298AC were not correlated with H. pylori infection in gastric cancer (P>0.05). Conclusion The polymorphisms in MTHFR 1298AC may be associated with the reduced risk of developing gastric cancer of antrum, but may not be associated with H. pylori infection.