焦炉工血清ras P21水平和外周血淋巴细胞SCE率检测

目的:为了解接触焦炉逸散物职业性人群遗传效应的改变,从分子水平揭示焦炉逸散物的遗传毒作用.方法:对25名焦炉工和12名正常对照分别检测了血清P21和外周血淋巴细胞姐妹染色单体互换(SCE)率.结果:发现焦炉工P21水平(3 774.2)、SCE率(11.43)明显高于对照组P21水平(1 270.7)和SCE率(6.37);焦炉工中,炉顶工血清P21均值(4 377.5)高于其它工种(3 300.1),炉顶工SCE(13.51)明显高于其它工种(10.07);对焦炉工按是否吸烟进行比较,未发现组间P21和SCE有差异显著性.结论:提示焦炉逸散物具有较强的遗传毒理学效应,可提高机体癌蛋白P21水平和SCE频率.

Detection of serum ras P21 level and sister chromatid exchange frequency in peripheral blood lymphocyte in workers exposed to coke oven volatiles

Aim: To investigate genotoxic effects in workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs) such as benzo-(a)-pyrene (BaP) in coke oven volatiles (COV). Methods: 25 workers exposed to COV (exposed group) and 12 health donors (normal group) were studied.Serum P21 levels were detected by Western Blotting. Sister chromatid exchange (SCE) frequencies were measured by culturing with peripheral blood lymphocyte. Results: Serum P21 levels and SCE/cell frequencies in exposed group were significantly higher than those of normal group (P21 (3 774.2±1 494.2)vs(1 270.7±623.7 and SCE(11.43±2.11) vs (6.37±0.59), respectively). P21 levels and SCE frequencies in workers working at coke oven top were remarkably higher than those of workers in other working environments (P21(4 377.5±1 632.7) vs (3 300.1±1 233.3) and SCE(13.51±1.76) vs (10.07±0.44), respectively). There was no significant difference in either P21 levels or SCE between smokers and nonsmokers (P >0. 05) in exposed group. Conclusion: Occupational exposure to COV resulted in an increased level of SCE/cell, P21. The frequency of SCE and the P21 levels were found to be related to exposure to carcinogenic PAHs. The results imply that COV have a potent genotoxic effect.