Partial N-terminal amino acid sequences of three nonstructural proteins of two flaviviruses |
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| Authors: | C M Rice R Aebersold D B Teplow J Pata J R Bell A V Vorndam D W Trent M W Brandriss J J Schlesinger J H Strauss |
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| Institution: | 1. Division of Biology, California Institute of Technology, Pasadena, California 91125, USA;2. Vector-Borne Virus Diseae Division, Centers for Diseae Control, P.O. Box 2087, Fort Collin, Colorado 80522, USA;3. Department of Medicine, Rochester General Hospital and the University of Rochester School of Medicine and Dentistry, Rochester, New York 14621, USA |
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| Abstract: | Partial N-terminal amino acid sequences for the three largest nonstructural proteins of two flaviviruses, yellow fever virus and St. Louis encephalitis virus, have been obtained. The determined sequences of these proteins exhibit significant amino acid sequence homology, and allow the positioning of these three nonstructural proteins in the polyprotein sequence deduced from the nucleotide sequence of yellow fever virus (C. M. Rice, E. M. Lenches, S. R. Eddy, S. J. Shin, R. L. Sheets, and J. H. Strauss, 1985, Science 229, 726-733.) The deduced start points support the hypothesis that the N terminus of nonstructural glycoprotein NS1 results from cleavage by signalase, whereas the N termini of NS3 and NS5 result from cleavages following double basic residues that are flanked by amino acids with short side chains. |
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