乳铁蛋白对大鼠上颌扩弓及复发过程中腭中缝骨吸收的影响及机制探究

目的 研究乳铁蛋白(lactoferrin,LF)对大鼠上颌扩弓及复发过程中腭中缝破骨活动的影响,并初探其作用机制.方法 对大鼠扩弓及复发模型给予剂量为1g/kg/d的LF灌胃,用免疫组织化学染色的方法观察LF对腭中缝破骨活动的影响,检测OPG/RANKL/RANK作用轴关键因子的表达变化.结果 与单纯扩弓组相比,LF...

Study of the mechanism of the effectiveness of lactoferrin on the mid-palatal suture bone resorption during the palatal expansion and relapse process

Objective To evaluate the effects of lactoferrin(LF)on rat bone resorption of the mid-palatal suture during maxillary expansion and relapse,and to preliminarily explore the mechanism.Methods The rat maxillary expansion and relapse model was gavaged daily with LF at the dose of 1 g/kg/d.Immunohistochemical staining was conducted to assess the effects of LF on bone resorption of mid-palatal suture.The expressions of key factors in the osteoprotegerin(OPG)/receptor or activator of nuclear factor-KB ligand(RANKL)/RANK axis were detected.Results Compared to the expansion only group,bone resorption was inhibited in the mid-palatal suture of the LF intervention group,accompanied by significantly increased expression ratio of OPG/RANKL.Conclusion LF gavage at 1 g/kg/d can suppress rat bone resorption of the mid-palatal suture during maxillary expansion and relapse,probably by regulating the OPG/RANKL/RANK axis.