Distribution of alpha1L-adrenoceptors in canine prostate: characterization by quantitative autoradiography

Our aim was to determine the distribution of alpha1L-adrenoceptors in canine prostate by an autoradiographic technique using 3H]JTH-601 (an alpha1L-adrenoceptor antagonist) and 3H]JTH-601-G1 (an active metabolite of JTH-601). Prostates were removed from three male beagle dogs. Several slices of the specimens were incubated with 5 nM of 3H]JTH-601, 3H]JTH-601-G1 and 3H]tamsulosin (an alpha1A-adrenoceptor antagonist). For macroscopic autoradiography, visualization was performed using an imaging plate and image-analyser. To examine microscopic localization of binding sites, preparations were exposed, developed and fixed. Specific binding of 3H]JTH-601 and 3H]JTH-601-G1 was observed diffusely throughout the entire interstitium on macroscopic autoradiography. Specific binding of 3H]tamsulosin was also recognized although the binding was weaker than that of 3H]JTH-601. On microscopic autoradiograms, the grains of each ligand were mainly distributed on smooth muscle. These results indicate morphologically that specific binding sites of JTH-601 and JTH-601-G1 exist in canine prostate, suggesting the distribution of alpha1L-adrenoceptors in this tissue, in addition to alpha1A-adrenoceptors.