人卵泡液对小鼠胚胎生长发育影响的研究

目的：探讨人卵泡液对小鼠胚胎生长发育的影响。方法：在培养液中分别加入人卵泡液（卵泡液组）和人血清（血清组），观察和检测2细胞期鼠胚在体外发育至8细胞期、囊胚期以及鼠胚孵出的比率；用放射免疫方法测定人卵泡液和血清中表皮生长因子（EGF）含量。结果：卵泡液组有72.9%的2细胞期鼠胚能发育到8细胞期，血清组仅有48.0%到达8细胞期；继续培养显示，卵泡液组细胞分裂较快，有50.9%的鼠胚可发育至囊胚期，并有26.3%孵出，明显高于血清组（24.5%,6.9%)，两组比较，差异有显著性（P＜0.05)。卵泡液中EGF的含量为0.50μg/L，血清中为0.26μg/L，两组比较，差异有显著性（P＜0.05）。结论：卵泡液对早期胚胎发育有促进作用，在早期胚胎培养中，添加人卵泡液比添加人血清对提高早期胚胎培养质量更为有效。

The effect of human follicular fluid on mouse embryo culture

OBJECTIVE: To evaluate the effect of human follicular fluid (HFF) on mouse embryo culture. METHODS: Compare the effects of HFF and serum as a protein supplement on the in vitro development of mouse embryos. Measure the concentration of the epidermal growth factor (EGF) in HFF and human serum. RESULTS: HFF significantly enhanced the developmental potential of mouse embryos in vitro, even in the presence of meiotic inhibitors. HFF significantly increased frequency of development from the 2-cell stage to the 8-cell stage (72.9%), blastocyst stage (50.9%) and hatching (26.3%), compared with human serum(48.0%, 24.5%, 6.9%, P < 0.05). There was higher concentration of EGF with an average of 0.50 microgram/L in HFF group compared with human serum (0.26 microgram/L, P < 0.05). CONCLUSION: Human follicular fluid is effective in increasing the developmental potential of mouse embryos in vitro.