LHRH-PE40对人结肠癌细胞增殖抑制和凋亡的影响

目的：探讨一种新型免疫毒素LHRH-PE40与人结肠癌细胞系Lovo表面LHRH受体结合的特性，观察其对细胞增殖产生的抑制作用及检测细胞凋亡。 方法：用125Ⅰ标记法检测LHRH-PE40与Lovo结合的特异性; MTT比色法检测LHRH-PE40对胃癌细胞的杀伤活性,流式细胞仪检测细胞凋亡情况。 结果：人结肠癌细胞系Lovo细胞表面可见配基-受体特异性结合，LHRH-PE40对Lovo细胞的半数致死量为0.24 mg·L^-1，0.1～10.0 mg·L^-1LHRH-PE40作用于Lovo细胞，其凋亡明显（P<0.01）。 结论：结肠癌细胞Lovo细胞表面存在LHRH受体，LHRH-PE40对结肠癌细胞的增殖状态有明显的抑制作用及促凋亡作用。 LHRH-PE40,结肠肿瘤,细胞凋亡,受体;LHRH

Influences of LHRH-PE40 on proliferation inhibition and apoptosis of human colon carcinoma cells

Objective To research the binding specificity of new recombination toxin LHRH-PE40 and LHRH receptor on the surface of human colon carcinoma cell line Lovo and the mechanism of anti-proliferation,and determine the apoptosis.Methods Lovo cells were analysed by LHRH-PE40 marked with()~(125)Ⅰ;the cytocidal effect of the anti-tumor was evaluated by MTT assay,and the apoptotic rate was analysed by flow cytometry.Results Lovo cells had the binding of aglucone and receptor.Half lethal dose of human colon carcinoma cells Lovo with LHRH-PE40 was 0.24 mg·L~(-1).The apoptotic rate was increased when the LHRH-PE40 concentration was ranged from 0.1 to 10 mg·L~(-1)(P<0.01).Conclusion Human colon carcinoma cell line Lovo have LHRH receptors.LHRH-PE40 can inhibit the colon carcinoma cell proliferation and induce the apoptosis.