Development and characterization of 21 polymorphic microsatellite markers for the barren-ground shrew, Sorex ugyunak (Mammalia: Sorcidae), through next-generation sequencing, and cross-species amplification in the masked shrew, S. cinereus |
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| Authors: | S. A. Sonsthagen G. K. Sage M. Fowler A. G. Hope J. A. Cook S. L. Talbot |
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| Affiliation: | 1. US Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA
2. Museum of Southwestern Biology and Department of Biology, University of New Mexico, Albuquerque, NM, 87131, USA
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| Abstract: | We used next generation shotgun sequencing to develop 21 novel microsatellite markers for the barren-ground shrew (Sorex ugyunak), which were polymorphic among individuals from northern Alaska. The loci displayed moderate allelic diversity (averaging 6.81 alleles per locus) and heterozygosity (averaging 70 %). Two loci deviated from Hardy–Weinberg equilibrium (HWE) due to heterozygote deficiency. While the population did not deviate from HWE overall, it showed significant linkage disequilibrium suggesting this population is not in mutation-drift equilibrium. Nineteen of 21 loci were polymorphic in masked shrews (S. cinereus) from interior Alaska and exhibited linkage equilibrium and HWE overall. All loci yielded sufficient variability for use in population studies. |
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