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Isolation and characterization of thirty microsatellite loci for Atlantic mackerel (Scomber scombrus L.)
Authors:Gudbjorg Olafsdottir  Kristinn Olafsson  Sigurlaug Skirnisdottir  Gudmundur J. Oskarsson  Doreen Kohlbach  Helga Franklinsdottir  Cecilia Elisabeth Klitgaard Kvaavik  Renée Morneau  André Chevrier  Christophe Pampoulie  Sarah Helyar  Anna K. Danielsdottir
Affiliation:1. Matís Ltd., Vínlandsleie 12, 113, Reykjavík, Iceland
2. Marine Research Institute, Skúlagata 4, 101, Reykjavík, Iceland
3. Fisheries and Oceans Canada, Quebec Region, Maurice Lamontagne Institute, 850, route de la Mer, P.O. Box 1000, Mont-Joli, QC, G5H 3Z4, Canada
Abstract:Atlantic mackerel (Scomber scombrus L.) is a widely distributed commercially important pelagic species. Little is known about the stock structure of this species, but it is thought to be undergoing a range extension due to environmental changes. Knowledge of the stock structure under these changing conditions is fundamental for effective management. In this paper, 30 highly polymorphic microsatellite loci developed with next generation sequencing are described. The number of alleles per locus ranged from 4 to 39 in two geographically distant populations, observed and expected heterozygosities ranged between 0.370–0.978 and 0.426–0.962, respectively. These loci are an important resource that will allow assessment of the current population genetic structure of this species, and enable monitoring of climate related changes in the species range and distribution.
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