低剂量X射线对淋巴细胞及各亚群细胞功能的刺激作用

作者在研究低剂量辐射对全淋巴细胞刺激性效应的同时，用３种单克隆抗体分离出分化抗原簇＋－４，分化抗原簇＋－８和Ｂ细胞亚群，进一步探讨了低剂量辐射对３种亚群细胞的刺激作用。结果表明，Ｐａｎｎｉｎｇ法分离的各亚群细胞的纯度及存活率均在９０％以上；全淋巴细胞在０．１Ｇｙ剂量范围内有刺激作用，尤以０．１Ｇｙ剂量点效应最大；各亚群细胞在０．２Ｇｙ照射内均有刺激性效应的发生．分化抗原簇＋－４和Ｂ细胞在０．１Ｇｙ剂量点效应最强，而分化抗原簇＋－８在０．０５Ｇｙ点效应最强；相同剂量，分化抗原簇＋－４的刺激效应大于分化抗原簇＋－８；０．５Ｇｙ照射则表现出明显的抑制作用．

Stimulative effect of low dose X-rays on lymphocytes and their subsets

Three kinds of McAbs (monoclonal antibody ) were used to separateCD_4, CD_3 and B cells by "panning"method .After low dose (0-0.5Gy)X-ray irradiation, the effects oftotal lymphocytes, CD_4, CD_3 and Bcells were studied using ~3H-TdRincorporation method. The resultsindicated that all the purities andsurvivals of CD_4, CD_3 and B subsetsseparated by McAbs were above90%; X-ray irradiation below 0. 1Gycould induce stimulative effect on total lymphocytes, especially at the doseof 0. 1Gy(139. 2%);0. 2Gy X-ray couldstill induce stimulative effects onthese three subsets .For CD_4, CD_3 andB cells,the greatest effects were induced by 0.1, 0.05 and 0.1Gy X-raysrespectively. For the same dose,the stimulative effect on CD_4 wasgreater than that on CD_3. Obviouslyinhibitory effects were observed after0.5 Gy X-ray irradiation.