快速起搏大鼠原代培养心房肌细胞内皮素-1表达的变化（英文）

目的：摘要：目的 ：利用原代培养的心房肌细胞建立快速起搏模型,研究在快速起搏早期内皮素-1（ET-1）表达的变化。方法：以原代培养大鼠心房肌细胞,建立快速起搏细胞模型,利用酶联免疫吸附（ELISA）法和逆转录聚合酶链式反应（RT-PCR）方法检测在快速起搏3、6、12、24 h后内皮素-1的mRNA表达和分泌量的变化。结果：与起搏前比较,快速起搏6 h后ET-1的mRNA表达[（0.31±0.02）比（0.52±0.07）、（0.62±0.09）、（0.75±0.05）]和分泌量[（3.4±0.8）ng/L比（6.0±1.4）ng/L、（8.3±1.5）ng/L、（11.2±2.1）ng/L]持续增加（P均〈0.01）。结论：快速起搏早期,原代培养心房肌细胞内皮素-1的mRNA表达和分泌量随起搏时间增加,提示内皮素-1参与了房颤时心房病理重构。

Expressions of endothelin-1in primary cultured atrial myocytes during early rapid pacing

Objective : To study the expressions of endothelin-1 in primary cultured atrial myocyte model at early stages of atrial fibrillation by rapid pacing.Methods : The primary rat atrial myocytes were cultured,in which a rapid paced cell model was established.The polymerase chain reaction(RT-PCR) and enzyme-linked immunosorbent assay(ELISA) were applied to detect the messenger ribonucleic acid(mRNA) expression and secretion of endothelin1 on 3h,6h,12h and24h after rapid pacing.Results : Compared with before pacing,there were continuous and significant increase in expression of ET-1mRNA [(0.31±0.02) vs.(0.52±0.07),(0.62±0.09),(0.75±0.05)] and secretion of endothelin-1 [(3.4±0.8) ng / L vs.(6.0±1.4) ng / L,(8.3±1.5) ng / L,(11.2±2.1) ng / L ] on 6h,12h and 24h after rapid pacing,P<0.01 all.Conclusion : Expressions of ET-1mRNA and secretion of endothelin-1 significantly increase depend on time in early rapid pacing atrial myocytes,indicating that endothelin-1 participates in atrial pathological remodeling during atrial fibrillation.