Restriction map and clone bank of tomato plastid DNA |
| |
| Authors: | Andy L. Phillips |
| |
| Affiliation: | (1) Department of Botany, University of Cambridge, CB2 3EA Cambridge, UK;(2) Present address: Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ Harpenden, UK |
| |
| Abstract: | Summary Tomato plastid DNA has been isolated from leaf chloroplasts andPst1 fragments cloned in the plasmid vector pUC8. Recombinant plasmids containing all but the largestPst1 fragment were identified by colony hybridisation. A restriction map of the plastid DNA for four restriction endonucleases was generated by digestion of the clonedPst1 fragments and by hybridisation to Southern blots. The plastid DNA is a circular molecule of 156.6–159.4 kbp with a large inverted repeat, and shows high conservation of restriction sites with plastid DNA from tobacco andPetunia. |
| |
| Keywords: | Tomato Plastid DNA Restriction map Clone bank |
| 本文献已被 SpringerLink 等数据库收录！ |
|
