C-myc小干扰RNA对HL-60细胞凋亡的影响

目的:研究c-myc小干扰RNA对白血病细胞凋亡的影响,探寻白血病基因治疗的新方法。方法:应用针对c-myc的小干扰RNA转染HL-60细胞48 h后,倒置显微镜下观察细胞形态学变化,琼脂糖凝胶电泳法检测DNA梯形带和流式细胞仪Annexin V-FITC/PI法检测细胞凋亡。结果:c-myc小干扰RNA作用后,HL-60细胞生长受抑,可见细胞核固缩,胞桨内出现凋亡小体。阴性对照组和空白对照组没有明显变化。DNA电泳干扰组出现典型的梯状条形带,对照组未出现明显凋亡梯形带。流式细胞仪分析干扰组细胞凋亡率可达26.21±0.75%,明显高于阴性对照组的4.58±0.48%和空白对照组的3.76±0.30%。结论:c-myc小干扰RNA对HL-60细胞有明显的抑制作用,可抑制细胞增殖,导致细胞凋亡,有望成为白血病靶向基因治疗的新工具。

The effect of c-myc small interference RNA on apoptosis of HL-60 cells

Objective: To study the effect of the c-myc small interference RNA on apoptosis of leukemic cells.Methods: After c-myc siRNA was transfected into HL-60 cells for 48 hours,growth apperance and morphology of the cells were observed with microscope,agarose gel electrophoresis was performed for the DNA ladder and the percentage of apoptosis cells was analyzed by flow cytometric annexin V-FITC/ PI.Results: After being acted by c-myc siRNA,the growth of HL-60 cells was inhibited,the nuclei of the cells shrinked and the apoptosis body was observed in the plasma of the cells.The DNA ladder was clearly detected in agarose gel electrophoresis,FCM analysis showed that the percentage of apoptosis cells in c-myc siRNA group was 26.21±0.75 %,significantly higher than that in control siRNA group 4.58±0.48 % and non-transfection group3.76±0.30%.Conclusion: c-myc siRNA could significently inhibit HL-60 cells,it inhibits HL-60 cells to proliferate and causes the cells to apoptosis.c-myc siRNA may become one of the new tools of targeting gene therapy to leukemia.