| 金银花多糖的提取、精制及含量测定 |
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| 引用本文： | 刘光海.金银花多糖的提取、精制及含量测定[J].湖南中医药大学学报,2009,29(11):33-34. |
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| 作者姓名： | 刘光海 |
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| 作者单位： | 黔东南州中医医院药剂科,贵州,凯里,556000 |
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| 摘 要： | 目的提取金银花中的多糖，精制并进行含量测定。方法采用热水法提取、乙醇沉淀制备粗多糖；Sevag法去蛋白制备精制多糖；苯酚-硫酸法测定多糖含量。结果粗多糖和精制多糖中多糖的含量分别为61．27％和80．54％。结论得到了高纯度的精制多糖。
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| 关 键 词： | 金银花 多糖 精制 含量测定 |
The Extraction, Purification and Content Determination of Polysaccharide from Flos Lonicerae Japonicae |
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| LIU Guang-hai.The Extraction, Purification and Content Determination of Polysaccharide from Flos Lonicerae Japonicae[J].Journal of Traditional Chinese Medicine University of Hunan,2009,29(11):33-34. |
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| Authors: | LIU Guang-hai |
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| Institution: | LIU Guang-hai (Qiandongnan Chinese Medicine Hospital Pharmacy, Kaili,Guizhou,556000,China) |
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| Abstract: | Objective To extract and purify polysaccharide of Ligustrum lucidum and determine carbohydrate content. Methods The crude polysaccharide was obtained by hot water extraction and ethanol precipitation. The crude polysaccharide was deproteinized by Scrag method.The relative percentage content of Flos Lonicerae polysaccharide was studied with sulfuric acid-phenol method. Results The carbohydrate content of crude polysaccharide and purified polysaccharide was 61.27% and 80.54%. Conclusion A purified polysaccharide was obtained from Flos Lonicerae Japonicae. |
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| Keywords: | Flos Lonicerae Japonicae polysaccharide purification content determination |
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