我国狂犬病病原学和病理形态学观察

报告四例狂犬病病例及对其尸脑组织的病原学和病理形态学观察。４例病人均有被犬咬伤史；发病后临床病状典型，表现为发热、恐水、怕风等；潜伏期和病程分别在２０～１３２天和７～１４天；临床诊断明确。死亡后尸检脑组织病理改变为病毒性脑炎病变，在感染神经细胞胞浆内均检到内基氏体。从尸脑组织中分离出狂犬病毒４株。又从４例病人血清中和２例病人脑脊液中分别检测出特异性狂犬病毒抗体各１例。

Rabies in Chongqing and Its Etiological Research

This paper reported 4 cases of patients clinically diagnosed as rabies with typical signs. Before the clinical signs manifested all patients had been bitten by the suspected rabid dogs. Detection of RV specific antibodies in both the blood and the CSF by Immunofluorescent focus inhibition test was carried out. The results showed that only one out of four cases was positive in blood while one of two cases was positive in CSF. Histopathological examinations on the postmortem brain sections suggested viral encephalitis resulting in an extensive neuronal invasion with limited cell destruction. However, Negri bodies might be demonstrated within the cytoplasm of neurons. If the sections stained with indirect immunofluorescence method, the RV-antigenic fluorescent particles could be detected in the brain tissues as well as within cytoplasm of neurons. Four virus strains were isolated from the postmortem brains of 4 cases by injection into mice intracerebrally. The patterns of the antigenic fluorescent particles in the mouse brain sections and the charecteristic ultramorphology of these viruses in thin sections were similar to those of the reference Beijing strain.