DNA病毒与RNA病毒相互作用小鼠模型的建立

用Ｃ型病毒（ＲＮＡ病毒）和／或紫外线灭活的（疱疹病毒）ＨＳＶ－２（Ｗｕ）或ＨＳＶ－２（３３３）接种新生小鼠，结果表明接种了ＨＳＶ－２（Ｗｕ）、ＨＳＶ－２（３３３）和作为对照的小鼠均未发生白血病，在ＨＳＶ－２（Ｗｕ）组中接种了Ｃ型病毒加ＨＳＶ－２（Ｗｕ）的小鼠白血病发生率是７５００％，只接种了Ｃ型病毒的小鼠中有４４．７４％发生白血病，两者之间有高度显著性差异（Ｐ＜０．０１）。另外，在ＨＳＶ－２（３３３）组中用Ｃ型病毒加ＨＳＶ－２（３３３）接种小鼠以后有７９．１６％发生了白血病，只接种了Ｃ型病毒的小鼠中有４１．０２％发生白血病，其差异也有高度显著性（Ｐ＜０．０１）。由此可见Ｃ型病毒分别与ＨＳＶ－２（Ｗｕ）或ＨＳＶ－２（３３３）在诱发小鼠白血病过程中存在相互作用。此动物模型可用于研究两类不同病毒相互作用的致癌原理。

Establishment of A Murine Model for DNA-RNA Virus Interaction

Herpesvirus is a DNA virus and type C virus is a RNA virus. In this experiment type C virus and / or UV-inactivated HSV-2 (Wu) or HSV-2 (333) were inoculated into newborn mice. The results showed that there was no leukemia to be found in mice inoculated with HSV-2 (Wu) or HSV-2 (333) and in controls. In HSV-2 (Wu) group the leukemia incidence in mice received type C virus plus HSV-2 (Wu) was 75.00%. and that in mice received type C virus alone was 44.74%. There was a highly significant difference between them (P < 0.01). In addition, in HSV-2 (333) group the leukemia incidence in mice inoculated with type C virus plus HSV-2 (333) was 79. 16%, and that in mice inoculated with type C alone was 40.02%. There was a highly significant difference between both rates (P< 0.01). This showed that there were interactions between type C and HSV-2 (Wu) or HSV-2 (333) during the inducement of leukemia in mice. This animal model might be used for studying the mechanism of interactions between two different classes of viruses.