中国人群9号染色体10个STR基因座的遗传多态性及其在法医学中的应用

目的：了解９号染色体１０个ＳＴＲ基因座在中国汉族群体的遗传多态性及其法医学应用价值。方法：无血缘关系的８３份个体样本采自甘肃省白银地区汉族群体。１８个无关汉族家系共１３１份血样采自同一地区。ＰＣＲ扩增９号染色体１０个ＳＴＲ基因座。ＰＣＲ产物由非变性聚丙烯酰胺凝胶电泳分析。结果：１０个ＳＴＲ基因座在中国汉族群体均具有遗传多态性。家系调查结果表明，１０个ＳＴＲ基因座在世代传递中遵循孟德尔常染色体共显性遗传规律。结论：为人类群体遗传研究提供了９号染色体１０个ＳＴＲ基因座的中国汉族群体数据。

Genetic polymorphism of 10 STR loci on chromosome 9 and its forensic application in Chinese population

OBJECTIVE: To have a thorough knowledge of the genetic polymorphism of 10 short tandem repeats loci of chromosome 9 in Chinese population and its application in forensic science. METHODS: The 9 STR tetrameric loci and one trimeric tandem repeat locus were chosen from Genome Databank. Eighty-three EDTA-blood samples were collected from the unrelated family in Baiyin city, Gansu province. DNA was extracted by Chelex method and amplified by the polymerase chain reaction(PCR). The PCR products were analyzed by PAGE electrophoresis. RESULTS: The polymorphisms of all 10 STR loci have been obtained in Chinese Han population. The 10 STR loci follow the Mendel ns law. CONCLUSION: The data of all 10 STR loci is beneficial to understanding the population genetics of them in Chinese Han population.