乙型肝炎患者外周血单个核细胞凋亡的检测及意义

目的研究激活诱导细胞死亡 (AICD)现象在乙型肝炎慢性化和重型化机制中的意义。方法分离 2 0例慢性 /慢性重型乙型肝炎病人与 10例健康献血员外周血单个核细胞 (PBMC) ,在 PHA的刺激下培养 72 h后收集细胞 ,经流式细胞仪检测凋亡。结果 PBMC凋亡率乙型肝炎组明显高于正常对照组 (2 5 .48± 14.0 7) % vs(11.45± 5 .2 7) % ,P<0 .0 1];慢性乙型肝炎组 (30 .5 7± 13.43) % ]明显高于正常对照组 (11.45± 5 .2 7) % ,P<0 .0 1]和慢性重型乙型肝炎组 (13.5 9± 6 .44 ) % ,P<0 .0 1];PBMC凋亡率乙型肝炎 HBe Ag(+ )组明显高于正常对照组 (2 9.5 0± 12 .5 4) % vs(11.45± 5 .2 7) % ,P<0 .0 1]。结论 AICD可能是形成 HBV慢性感染免疫耐受的一个重要机制。

Apoptosis of peripheral blood mononuclear cells of patients with hepatitis B and its significance

Objective To study the significance of AICD(activation-inducedcell death) in the pathogenesis of chronic hepatitis B and chronic severe hepatitis B. Methods Peripheral blood mononuclear cells(PBMCs)were isolated from 10 healthy blood donors and 20 patients with chronic/chronic severe hepatitis B. PBMCs were cultured with PHA-P for 72 hours in vitro. Then apoptosis of the PBMC was assayed by flow cytometry.Results The percentage of PBMC apoptosis of hepatitis B group was significantly higher than that of normal control group (25.48±14.07）％ vs （11.45±5.27）%,P<0.01]. The percentage of PBMC apoptosis of chronic hepatitis B group(30.57±13.43)%] was also significantly higher than that of normal control group（11.45±5.27）%, P<0.01]and higher than that of chronic severe hepatitis B group(13.59±6.44）%, P<0.01]. The percentage of PBMC apoptosis of hepatitis B with HBeAg(+) group was significantly higher than that of normal control group (29.50±12.54）% vs （11.45±5.27）%], P<0.01].Conclusion The results show that AICD may be an important mechanism of peripheral immune tolerance in HBV chronic infection.