慢性乙型肝炎急性发作患者血清病毒准种动力学研究

目的揭示慢性乙型肝炎急性发作及重型化过程中血清乙型肝炎病毒(HBV)基因组前C／C 及基本核心启动子(BCP)区准种的动态变化规律。方法选取1例慢性乙型肝炎两次急性发作伴重型化患者病程中5份血清样本,采用聚合酶链反应-TA克隆-构象敏感凝胶电泳-测序的方法,研究慢性乙型肝炎重型化过程中血清HBV准种的动态变化。结果血清HBV准种复杂性在慢性乙型肝炎急性发作时为10～ 12,在好转时为14～17(t=3．133,P<0．05),且随时间推移有逐渐升高的趋势;在此过程中优势准种组成及比例亦发生了变化(t=3．295,P

Study of the quasispecies dynamics of serum hepatitis B virus in a patient with acute exacerbations of chronic hepatitis B

Objective To investigate the quasispecies dynamics of hepatitis B virus (HBV) during the course of exacerbation and resolution of chronic hepatitis B in a patients. Methods Five serum samples were collected from a patient with two episodes of exacerbation and resolution of chronic hepatitis B. A method of PCR-TA cloning-conforma-tion sensitive gel electrophoresis (CSGE)-sequencing was performed to study the dynamic changes of HBV quasispecies in basal core promoter (BCP), precore and core regions of HBV genome. Results Quasispecies complexity was 10 and 12 at the points of exacerbations, and 14 and 17 at the points of resolutions (t = 3.133, P < 0.05). Ratio of dominant quasispecies in HBV population was high (42.4% and 51.5%) during exacerbations and low (30.3% and 21.2%) during resolutions (t = 3.295, P < 0.05). All dominant quasispecies, except the one during the second resolution, carried core P5T, L60V, S155T, and precore G1896A mutations. Conclusion The composition of HBV quasispecies changes due to the change of host immune status, and immune pressure might lead to the selection of immune escape mutants.