严重腹腔感染时胃粘膜Na+-K+-ATPase活性与电位差的相关性研究

目的：探讨严重腹腔感染状态下大鼠胃粘膜Na^ -K^ -ATPase活性变化对胃粘膜电位差(GTPD)的影响。方法：利用大鼠盲肠结扎穿孔造成腹腔严重感染动物模型，应用电生理记录仅检测穿孔前和穿孔后3h、6h、12h、24h、48hGTPD变化；应用生化法到定了各时相大鼠胃粘膜Na^ -K^ -ATPase活性。结果：盲肠穿孔后Na^ -K^ -ATPase活性显著降低(P＜0．05)，12h降至最低(P＜0．01)，仅为对照组的48．5％，48h仍未恢复正常。GTPD伤后6h显著下降(P＜0．05)，12h降至最低(P＜0.01)，24h和48h仍显著低于对照组(P＜0.05，P＜0．05)。结论：严重腹腔感染状态下胃粘膜Na^ -K^ -ATPase活性降低可能是引起胃粘膜屏障功能受损的主要原因。

INTERRELATED STUDY OF GASTRIC MUCOSAL NA+-K+- ATPASE ACTIVITY AND GASTRIC MUCOSAL TRANSMUCOSAL POTENTIAL DIFFERENCE DURING SEVERE INTRAPERITONEAL INFECTION IN RATS

Objective:To investigate the effect of gastric mucosal Na +-K +-ATPase activity on gastric transmucosal potential difference during severe intraperitoneal infection in rats.Methods: The intraperitoneal infection rat model was established by cecal ligation and puncture(CLP). The electric-physiological recorder meter was used to measure gastric mucosal potential difference. Assay of Na +-K +-ATPase activity in gastric mucosal tissue was conducted by biochemistry method. Results:The activity of Na +-K +-ATPase was markedly decreased in infected group at 3h after perforation, compared with the control group ( P <0.05) . There was a minimum of Na +-K +-ATPase activity at 12h post-perforation in infected group ( P <0.01), only about 46.6% matched to the control group. Gastric transmucosal potential difference (GTPD) of infected group decreased significantly compared with the control group ( P <0.05) at 6h after perforation, and rapidly dropped to the lowest at 12h post-perforation ( P <0.01). At 24h and 48h after perforation, GTPD was lower in infected group than that in the control group ( P <0.05, P <0.05). Conclusion:The reduce of Na +-K +- ATPase activity might be play an important role in gastric mucosal barrier damage following severe abdominal infection induced by CLP.