P33ING1b和KAI1/CD82在贲门癌组织中的表达及其意义

目的:探讨抑癌基因P33INGlb和KAI1/CD82在贲门癌(GCA)组织中的表达及二者在GCA的发生和恶性进展中的作用。方法:选取20例成人正常贲门黏膜(正常组)和51例GCA组织(GCA组),采用免疫组织化学方法检测2组组织中P33INGlb和KAI1/CD82蛋白表达的阳性率,结合临床病理资料进行统计学分析。结果:GCA和贲门黏膜细胞的胞浆见KAI1/CD82蛋白阳性表达颗粒。正常贲门黏膜组织中KAI1/CD82蛋白表达的阳性率为85.0%,GCA组织中KAI1/CD82蛋白表达的阳性率为65.7%。伴淋巴结转移患者GCA组织中的KAI1/CD82蛋白表达水平明显低于无淋巴结转移者(P<0.05);侵透浆膜患者的GCA组织中KAI1/CD82蛋白表达水平明显低于未侵及浆膜者(P<0.05)。KAI1/CD82蛋白表达的降低或缺失与肿瘤的临床分期有关联(P<0.05)。GCA和贲门黏膜细胞的胞浆和胞核见P33INGlb蛋白阳性表达颗粒。GCA组织较正常贲门黏膜组织的P33INGlb蛋白阳性表达率降低(P<0.05);P33INGlb蛋白表达的降低或缺失与肿瘤的临床分期有关联(P<0.05)。结论:KAI1/CD82蛋白在GCA组织的表达与肿瘤的临床分期、浸润深度和淋巴结转移有关联;GCA组织中P33INGlb蛋白表达率与肿瘤的临床分期有关;抑癌基因P33INGlb和KAI1/CD82在GCA组织中的表达可作为从分子水平判定人GCA恶性生物学行为的指标。

Expressions of P33ING1b and KAI1/CD82 in gastric cardia carcinoma tissue and their significances

Objective To study the expressions of P33ING1b and KAI1/CD82in gastric cardia carcinoma(GCA) tissue and their roles in the occurrence and malignancy progress of GCA.Methods 20 cases of adult normal cardia mucosa tissue(normal group) and 51 cases of GCA tissue(GCA group) were selected.Immunohistochemistry method was used to detect the positive expression rates of P33INGlb and KAI1/CD82 in the tissue in two groups.The data combined with the clinicopathological features was analyzed.Results KAI1/CD82 protein mainly expressed in the cytoplasm of GCA cells and cardia mucosal cells.The positive expression rate of KAI1/CD82 protein in normal gastric cardia tissue was 85.0%,and the positive expression rate of KAI1/CD82 protein in GCA tissue was 65.7%.The expression level of KAI1/CD82 protein in GCA tissue with lymph node metastasis was lower than that in GCA tissue without lymph node metastasis(P<0.05).The expression level of KAI1/CD82 protein in GCA tissue with serosa invasion was lower than that in GCA tissue without serosa invasion(P<0.05).The loss or reduction of KAI1/CD82 protein expression had relationships with clinical grade of tumor(P<0.05).P33INGlb protein mainly expressed in the cytoplasm and nucleolus of GCA cells and cardia mucosal cells.The expression level of P33INGlb protein in GCA tissue was lower than that in normal gastric cardia mucosa tissue(P<0.05).The loss or reduction of P33INGlb protein expression had relationships with clinical grade of tumor(P<0.05).Conclusion The expression of KAI1/CD82 protein in GCA tissue has relationship with clinical grade,the depth of tumor invasion,and lymth metastasis.The expression rate of P33INGlb protein in GCA tissue has relationship with the clinical grade of tumor.The expressions of KAI1/CD82 protein and P33INGlb protein can be used as indicators to judge the malignant biological behavior of human GCA at molecular level.