C6脑胶质瘤模型大鼠MRI影像及形态学观察及其意义

目的:建立稳定可靠的大鼠C6胶质瘤模型，对模型动物脑瘤体进行磁共振成像（MRI）影像动态观察及病理形态学观察，为抗脑胶质瘤药效学研究提供实验基础。方法：选择10只健康成年雄性Wistar大鼠，用立体定向将20 μL含有1×106个C6胶质瘤细胞的悬液缓慢接种于大鼠左侧尾状核区；接种后对10只大鼠进行一般状况观察，并分别于接种后第2、3和4周进行MRI影像动态学观察，同时对自然死亡荷瘤大鼠脑组织进行大体和病理组织学检查，计算荷瘤大鼠生存期。结果：大鼠术后2周出现活动和进食量减少、皮毛污秽及结膜充血等表现；3周后，有的大鼠出现肢体抽搐、偏瘫、身体扭曲和自身旋转等神经系统症状，10只荷瘤大鼠分别于8~30 d自然死亡。本组荷瘤大鼠中位生存期为18 d，平均生存期为(18.3±7.3) d。荷瘤大鼠脑瘤体随着瘤龄的增加而增大，病理学检查显示肿瘤细胞排列混乱致密，核分裂相易见，肿瘤内可见有血管组织增生，肿瘤浸润性生长呈指状突入周边正常组织。肿瘤细胞胶质酸性纤维蛋白(GFAP)表达呈阳性。结论：采用立体定向技术成功建立大鼠C6胶质瘤动物模型，MRI动态及病理形态学观察结果为选择适当的时间窗进行抗胶质瘤药物药效学研究提供实验基础。

MRI and morphological observation in C6 glioma model rats and significance

Objective To establish stable and reliable rat C6 glioma model,and to perform MRI dynamic observation and pathomorphological observation in model animal brain,and to provide experimental basis for pharmaceutical research on anti-glioma drugs.Methods The C6 glioma cells were cultured and 20 μL cultural fluid containing 1×106 C6 cells was sterotactically implanted into the left caudate nuclei in 10 male Wistar rats,respectively.The changes in the behavior of the rats after implantation were observed and recorded.MRI dynamic scanning was performed in 10 rats 2,3 and 4 weeks after implantation and the brain tissues were taken for general and pathological examination when the 10 rats were naturally dead.The survival period of tumor-bearing rats was calculated.Results 2 weeks after implantation the rats showed decreased activities and food intake,fur lackluster,and conjunctival congestion and so on;3 weeks later,some rats appeared nerve symptoms such as body twitch,body hemiplegy,body distortion,rotation and so on.All the 10 rats died in 8-30 d.The median surrival period of the tumor-bearing rats was 18 d,the average survival period was(18.3±7.3) d.The pathological examination showed that the tumor cells were arranged irregularly and closely and karyokinesis was easy to see;tumor vascular tissue proliferation and tumor invasive growth into surrounding normal tissues were found.The expression of glial fibrillary acidic protein(GFAP) was positive in the tumors.Conclusion A stable animal model of intracranial glioma is sucessfully established by stereotactic implantation of C6 cells into the rat caudate nucleus.The results of MRI dynamic observation and pathohistological observation on the model animal brain tissue.can provide experimental basis for selecting the appropriate time window to perform the pharmaceutical research on anti-glioma drugs.