VDR基因FokⅠ多态性与不同严重程度前列腺炎发生的关联性分析

目的：研究维生素D受体(VDR)基因启动子FokⅠ单核苷酸多态性(SNP)与不同严重程度前列腺炎（HP）的关联性，探讨遗传学因素在HP发病中的作用。方法：选择169例良性前列腺增生（BPH）并发HP患者，采用常规病理学检查将患者分为轻度组79例，中度组52例，重度组38例，应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法进行 FokⅠ SNP检测，分析3组患者基因型和等位基因频数分布情况。结果：轻、中度HP患者Fok Ⅰ位点3种基因型分布符合Hardy-Weinberg平衡定律(P>0.05)，重度HP患者中Fok Ⅰ位点3种基因型的分布不符合Hardy-Weinberg平衡定律(P<0.05)。FF、Ff和ff基因型在轻度和中度HP患者的分布为32%（25/79），46%（36/79）；22%（18/79），23%（12/52）；40%（21/52），37%（19/52）；组间比较差异均无统计学意义(P>0.05)。F基因频率在轻度和中度HP患者组分别为54%（86/158）和43%（45/104），f基因频率在2组间分别为46%（72/158）和57%（59/104），组间比较差异无统计学意义(P>0.05)。结论:VDR基因FokⅠ SNP可能与不同严重程度HP发生无关联。

Association analysis between vitamin D receptor gene polymorphism and occurrence of different degrees of histological prostatitis

Objective To investigate the association between single nucleotide polymorphism(SNP) of Fok Ⅰ in vitamin D receptor(VDR) gene and different degrees of histological prostatitis(HP),and to discuss the role of genetic factors in the onset of HP.Methods 169 patients with benign prostatic hyperplasia(BPH) complicated with HP were selected.The mild(79 cases),moderate(52 cases) and severe(38 cases) HP patients were distinguished by pathological examination.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to determine the SNP of Fok Ⅰ of patients.The distribution of genotypic and allelic frequencies of the patients in various groups was analyzed.Results The distribution of genotypic frequency of Fok Ⅰ site in mild and moderate HP patients didn't deviate from Hardy-Weinberg equilibrium(P>0.05).The distribution of genotypic frequency of Fok Ⅰ gene in severe HP patients deviated from Hardy-Weinberg equilibrium(P<0.05).The distribution of FF,Ff,ff genotypes in mild and moderate HP patients were 32%(25/79),46%(36/79);22%(18/79),23%(12/52);40%(21/52),37%(19/52),respectively;there was no significant difference between two groups(P>0.05).The allelic frequencies of F and f were 54%(86/158),46%(72/158) in mild HP patients and 43%(45/104),57%(59/104) in moderate HP patients,respectively;there was no significant difference between two groups(P>0.05).Conclusion VDR gene FokⅠ SNP may not associate with the occurrence of different degrees of HP.